Metabolome analysis of rice leaves to obtain low-oxalate strain from ion beam-mutagenised population
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ORIGINAL ARTICLE
Metabolome analysis of rice leaves to obtain low-oxalate strain from ion beam-mutagenised population Atsuko Miyagi1 · Takuya Saimaru1 · Nozomi Harigai2 · Yutaka Oono3 · Yoshihiro Hase3 · Maki Kawai‑Yamada1 Received: 31 March 2020 / Accepted: 18 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Introduction Rice leaves and stems, which can be used as rice straw for livestock feed, accumulate soluble oxalate. The oxalate content often reaches 5% of the dry weight leaves. Excess uptake of oxalate-rich plants causes mineral deficiencies in vertebrates, so it is important to reduce the oxalate content in rice leaves to produce high-quality rice straw. However, the mechanism of oxalate accumulation in rice has remained unknown. Objectives To understand metabolic networks relating oxalate accumulation in rice. Methods In this study, we performed metabolome analysis of rice M 2 population generated by ion-beam irradiation using CE-MS. Results The result showed wide variation of oxalate contents in M2 plants compared with those of control plants. Multivariate analyses of metabolome dataset revealed that oxalate accumulation was strongly related with anionic compounds such as 2OG and succinate. For low-oxalate plants, four patterns of metabolic alterations affected oxalate contents in the M 2 leaves were observed. In M3 plants, we found putative low-oxalate line obtained from low-oxalate M2 mutant. Conclusions These findings would lead to produce the low-oxalate rice and to understand the oxalate synthesis in plants. These findings would lead to produce the low-oxalate rice and to understand the oxalate synthesis in plants. Keywords Carbon ion beam · CE-MS · Metabolic alterations · Oryza sativa · Oxalate Abbreviations CE-MS Capillary electrophoresis-mass spectrometry DHAP Dihydroxyacetone phosphate G1P Glucose-1-phosphate Atsuko Miyagi and Takuya Saimaru have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11306-020-01713-y) contains supplementary material, which is available to authorized users. * Maki Kawai‑Yamada [email protected]‑u.ac.jp 1
Graduate School of Science and Engineering, Saitama University, 225 Shimo‑Okubo, Sakura‑ku, Saitama‑City, Saitama 338‑8570, Japan
2
Department of Life Environmental Chemistry, Saitama Institute of Technology, 1690 Fusaiji, Fukaya‑City, Saitama 369‑0293, Japan
3
Department of Radiation‑Applied Biology, National Institutes for Quantum and Radiological Science and Technology (QST), 1233 Watanuki, Takasaki‑City, Gunma 370‑1292, Japan
G6P Glucose-6-phosphate GABA Gamma-aminobutyrate FBP Fructose-1, 6-bisphosphate LET Linear energy transfer 2OG 2-oxoglutarate PC Principal component PEP Phosphoenolpyruvate 6PG 6-phosphogluconate 3PGA 3-phosphoglycerate RuBP Ribulose-1, 5-bisphosphate R5P Ribose-5-phosphate Ru5P Riburose-5-phosphate TCA Tricarboxylic acid
1 Introduction Rice is not used only as a major f
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