Optimization of Enzymatic Pretreatments to Obtain Fermentable Sugars from Fruit and Vegetable Waste
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ORIGINAL PAPER
Optimization of Enzymatic Pretreatments to Obtain Fermentable Sugars from Fruit and Vegetable Waste Michael A. Cabas Candama1 · Sara Duque Martinez1 · Edith M. Cadena Chamorro1 Received: 15 February 2019 / Accepted: 23 September 2019 © Springer Nature B.V. 2019
Abstract Biofuels production from organic waste requires an efficient hydrolysis to produce fermentable sugars. Physicochemical and biological pretreatment corresponds to good alternative to improve biofuel yield. Nonetheless, physical treatments can result in a negative energy balance and chemical treatments can generate inhibitors for a fermentative process, as well as difficulties in the recycling of organic matter. Thus, enzymatic pretreatments lead to fast and ecofriendly processes for conversion of waste biomass into monomeric units. In this work, fruit and vegetable wastes were hydrolyzed applying three types of enzymatic complexes: Viscozyme® L and a mixture of Multifect® B and Naturalzyme 40 XLTM. Production of reducing sugars as a temperature function and enzyme concentration was optimized by response surface analysis. Enzymatic complexes revealed high hydrolysis yield. Viscozyme® L application is highlighted; obtaining a hydrolysis higher than with the mixture of Multifect® B and Naturalzyme 40 XLTM (80% and 60% respectively) and being a promising treatment for the development of an efficient saccharification process of fruit and vegetable wastes. The optimum application conditions for Viscozyme® L were of 0.24 ppm and 40 °C and were validated with an error rate of 5%. For the mixture of Multifect® B and Naturalzyme 40 XLTM with Pectinase/Polygalacturonase activity, an optimum 0.93 ppm and 41 °C was found; these were validated with an error of 6%. Therefore, cellulases, xylanases and hemicellulases (Viscozyme® L) assured the hydrolysis of food wastes obtaining better available sugars for successive fermentative processes.
* Sara Duque Martinez [email protected] 1
Department of Agricultural Engineering and Food, Faculty of Agricultural Sciences, Universidad Nacional de Colombia - Sede Medellin, Carrera 65 N° 59A – 110, Medellin, Colombia
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Vol.:(0123456789)
Waste and Biomass Valorization
Graphic Abstract
Keywords Reducing sugars · Enzymatic pretreatments · Fruit and vegetable wastes · Hydrolysis yield
Statement of Novelty The use of agro-industrial waste and the concern to produce green energies has reached worldwide interest. Nowadays, lignocellulosic biomass is recycled to obtain biofuels, through a process that employs clean technologies and closed cycles. Various studies have shown that the physical and chemical treatments of organic material could reduce the time required for the hydrolysis, which is the rate limiting step in anaerobic digestion of organic waste. Nonetheless, the present study reveals that commercial enzymes can assure a high hydrolysis of complex polymers residues, by obtaining available sugars for successive fermentation processes.
Introduction Organic waste production requires the
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