Methyl jasmonate regulates protective enzyme activities to improve resistance to Venturia nashicola in pear ( Pyrus bret
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Methyl jasmonate regulates protective enzyme activities to improve resistance to Venturia nashicola in pear (Pyrus bretschneideri Rehd.) Shuyi Jing & Yi Liu & Huifang Liu & Na Li & Liulin Li
Received: 12 December 2019 / Revised: 16 June 2020 / Accepted: 12 August 2020 # Koninklijke Nederlandse Planteziektenkundige Vereniging 2020
Abstract Pear scab caused by Venturia nashicola is a devastating disease responsible for huge economic losses to the pear industry. The enhancement of pear resistance to scab by methyl jasmonate (MeJA) was evaluated in the susceptible cultivar ‘Xuehua’ and the resistant cultivar ‘Huangguan’ of Chinese pear (Pyrus bretschneideri Rehd.). The regulatory mechanism of MeJA was analyzed by measuring the activities of four protective enzymes and expression levels of corresponding genes. The results showed that MeJA inhibited the growth of V. nashicola on oat agar medium. The in vitro growth-inhibitory effect of MeJA was enhanced with increasing MeJA concentration over the Shuyi Jing and Yi Liu contributed equally to this work. S. Jing : H. Liu : N. Li : L. Li (*) College of Horticulture, Shanxi Agricultural University, ShanxiTaigu 030800, China e-mail: [email protected]
S. Jing e-mail: [email protected] H. Liu e-mail: [email protected] N. Li e-mail: [email protected] Y. Liu College of Horticulture, Shanxi Agricultural University (Horticulture Research Institute, Shanxi Academy of Agricultural), ShanxiTaiyuan 030031, China e-mail: [email protected]
range 0.05–2 mmol L− 1, and the difference was significant at 0.1 mmol L− 1 or higher concentration levels (p < 0.05). Except peroxidase, all the other enzymes exhibited higher activities in ‘Huangguan’ pear than in ‘Xuehua’ pear after inoculation. Treatment with 0.1 mmol L− 1 MeJA substantially enhanced all the protective enzyme activities in inoculated leaves of the two pear cultivars, with increased expression of related genes. These results indicate that MeJA improves resistance of pear to scab by increasing protective enzyme activities. Keywords ‘Huangguan’ pear . ‘Xuehua’ pear . Methyl jasmonate . Pear scab . Protective enzyme . Venturia nashicola
China is one of the centers of origin of pear (Pyrus L.), with a long cultivation history and extensive cultivation (Teng et al. 2004). Pear scab caused by Venturia nashicola Tanaka et Yamamoto is a devastating disease (Ishii and Yanase 2000), bringing huge economic losses to the pear industry (Ma 2014). Chemical control is currently the main measure for the prevention and treatment of scab in pear production; however, long-term use of chemical agents will increase resistance of pathogenic bacteria, cause pollution in orchards, and harm the health of consumers (Ishii 2004). It is, therefore, imperative to formulate more effective measures to control the development of pear scab. Induced resistance for plant defense has many advantages, such as a wide range of sources, broad-spectrum application, food safety, and
Eur J Plant Pathol
environmental protection. Thus, providing new ideas for the p
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