Methylmercury-Induced Toxicopathologic Findings in Salivary Glands of Offspring Rats After Gestational and Lactational E
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Methylmercury-Induced Toxicopathologic Findings in Salivary Glands of Offspring Rats After Gestational and Lactational Exposure Priscila Cunha Nascimento 1 & Maria Karolina Martins Ferreira 1 & Karolyny Martins Balbinot 2 & Sérgio Melo Alves-Júnior 2 & João de Jesus Viana Pinheiro 2 & Felipe Martins Silveira 3 & Manoela Domingues Martins 3 & Maria Elena Crespo-Lopez 4 & Rafael Rodrigues Lima 1 Received: 30 August 2020 / Accepted: 21 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Methylmercury (MeHg) is one of the main global pollutants. The vulnerability of fetus and newborn to MeHg-induced changes is extensively reported, making relevant investigation possible for alternative sample matrix for human biological monitoring for at this stage of life. This study aimed to characterize tissue change effects of environmental–experimental MeHg on salivary glands of offspring rats after pre- and postnatal exposure. For this, pregnant Wistar rats were orally exposed to MeHg (40 μg/kg BW/ day) or only vehicle (control group), from the gestational period to the end of the lactation period. Salivary glands (SG) were collected from the offspring to analyze possible Hg levels and main findings by histopathological evaluations and CK19 and αSMA immunostaining. The results indicated that Hg levels in SG of intoxicated offspring were associated with histologic abnormalities, such as acinar atrophy and an increase in the intercellular matrix among the acini, as well as damages in the architecture of epithelium and myoepithelial cells, evidenced by a decrease in immunostaining area. Thus, this is the first study to show in the literature the toxicopathologic findings on SG of offspring after pre- and postnatal exposure to MeHg. Moreover, it presents the SG as an attractive target to futures studies, mainly in children exposed to environmentally relevant doses. Keywords Methylmercury . Offspring . Salivary glands . Morphological findings
Introduction Methylmercury (MeHg) is a toxic organic compound widely distributed in the environment by natural and mainly anthropogenic sources of mercury release [1–3]. Currently, it remains a global public health issue due to environmentally Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12011-020-02409-z) contains supplementary material, which is available to authorized users. * Rafael Rodrigues Lima [email protected] 1
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Para, Rua Augusto Corrêa n° 1, Campus do Guamá, Belém, Pará 66075-110, Brazil
2
School of Dentistry, Institute of Health Sciences, Federal University of Pará, Belém, Pará, Brazil
3
Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
4
Laboratory of Molecular Pharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil
relevant levels of MeHg [3–5], coming from mostly artisanal and sma
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