Microarray, MicroRNA, and Angiogenesis
Angiogenesis is defined as the formation and the growth of new blood vessels that sprout from existing vascular network. Angiogenesis plays a very important role in the physiological and pathological situations such as ischemia, atherosclerosis, wound hea
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Microarray, MicroRNA, and Angiogenesis Zufeng Ding, Xianwei Wang, and Jawahar L. Mehta
Abstract Angiogenesis is defined as the formation and the growth of new blood vessels that sprout from existing vascular network. Angiogenesis plays a very important role in the physiological and pathological situations such as ischemia, atherosclerosis, wound healing, and cancer growth and metastasis. Microarray is a new molecular biological technique that can analyze expression of hundreds to thousands of genes or proteins simultaneously in a single experiment. Microarray includes DNA microarray, protein microarray, tissue microarray, and cell microarray. Many different types of microarray chips are now commercially available, some designed specifically for angiogenesis. MicroRNAs (miRNAs) are endogenous, short, noncoding RNAs found in eukaryotic cells. MiRNAs are posttranscriptional regulators that negatively regulate gene expression by binding to their target messenger RNAs for degradation and/or translational repression. The main function of miRNAs is gene regulation. MiRNAs have been found to modulate many pathophysiological process including cell differentiation, contraction, migration, proliferation, apoptosis, and tissue inflammation. There are more than 1,000 miRNAs in human genome, some of them are involved in angiogenesis. In this review, we will summarize the application of microarray assay and function of miRNAs in angiogenesis. Keywords Microarray • MicroRNA • Angiogenesis • Vascular endothelial growth factor
Zufeng Ding and Xianwei Wang contributed equally to this work Z. Ding • J.L. Mehta • X. Wang () Division of Cardiovascular Medicine, University of Arkansas for Medical Sciences and the Central Arkansas Veterans Healthcare System, 4301 West Markham, Little Rock, AR 72205, USA e-mail: [email protected]
J.L. Mehta and N.S. Dhalla (eds.), Biochemical Basis and Therapeutic Implications of Angiogenesis, Advances in Biochemistry in Health and Disease 6, DOI 10.1007/978-1-4614-5857-9_24, © Springer Science+Business Media New York 2013
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Abbreviations Ang bFGF CTGF ECs EGF EGFL7 eNOS FLT1 HGS HIF-1 IFN-γ IGF-1 MCP-1 MMPs MRE PDGF PIK3R2 PGF PE RCC RL ROS SCF SGA TGF-ß1 TIMP VEGF
24.1
Angiotensin Basic fibroblast growth factor Connective tissue growth factor Endothelial cells Endothelial growth factor Epidermal growth factor-like domain 7 Endothelial nitric oxide synthase FMS-related tyrosine kinase 1 Hepatocyte growth factor-regulated tyrosine kinase substrate Hypoxia-inducible factor-1 Interferon γ Insulin-like growth factor 1 Monocyte chemoattractant protein 1 Matrix metalloproteinases MicroRNA responsive element Platelet-derived growth factor Phosphoinositol-3 kinase regulatory subunit 2 Placental growth factor Preeclamptic Renal cell carcinoma Renilla luciferase Reactive oxygen species Stem cell factor Small-for-gestational-age Transforming growth factor ß1 Tissue inhibitor of metalloproteinase Vascular endothelial growth factor
Introduction
Angiogenesis is defined as the formation o
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