Microarray Technology Methods and Applications

This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs, proteins, glycans and whole cells. Written for the Methods in Molecular

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Paul C.H. Li · Abootaleb Sedighi Lin Wang Editors

Microarray Technology Methods and Applications

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Microarray Technology Methods and Applications

Edited by

Paul C.H. Li Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada

Abootaleb Sedighi Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada

Lin Wang Department of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada

Editors Paul C.H. Li Department of Chemistry Simon Fraser University Burnaby, BC, Canada

Abootaleb Sedighi Department of Chemistry Simon Fraser University Burnaby, BC, Canada

Lin Wang Department of Biomedical Engineering University of British Columbia Vancouver, BC, Canada

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3135-4 ISBN 978-1-4939-3136-1 (eBook) DOI 10.1007/978-1-4939-3136-1 Library of Congress Control Number: 2015952014 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)

Dedication Dedicated to Cindy Woo, Paul Chan who have encouraged and supported my quest for analytical tools to fight cancer, in Australia and beyond.

Preface The microarray method has been a widely applied technology. There are considerable amounts of technical and scientific literature published recently. The aim of this book is to provide updates of this established technology in topics such as methods and applications, as well as advances in applications of the microarray method to assay substances such as proteins, glycans, and whole cells. The