Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Proper
- PDF / 286,484 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 0 Downloads / 154 Views
Research Article Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Properties and Efficacy in Cutaneous HSV-1 Infection in Mice Pattaraporn Sasivimolphan,1 Vimolmas Lipipun,1,7 Garnpimol Ritthidej,2 Khanidtha Chitphet,1 Yoshihiro Yoshida,3 Tohru Daikoku,3 Boonchoo Sritularak,4 Kittisak Likhitwitayawuid,4 Pornpen Pramyothin,5 Masao Hattori,6 and Kimiyasu Shiraki3
Received 10 April 2012; accepted 16 July 2012; published online 2 August 2012 Abstract. The physicochemical properties of the optimized microemulsion and the permeating ability of oxyresveratrol in microemulsion were evaluated, and the efficacy of oxyresveratrol microemulsion in cutaneous herpes simplex virus type 1 (HSV-1) infection in mice was examined. The optimized microemulsion was composed of 10% w/w of isopropyl myristate, 35% w/w of Tween 80, 35% w/w of isopropyl alcohol, and 20% w/w of water. The mean particle diameter was 9.67±0.58 nm, and the solubility of oxyresveratrol in the microemulsion was 196.34±0.80 mg/ml. After accelerated and long-term stability testing, the microemulsion base and oxyresveratrol-loaded microemulsion were stable. The cumulative amount of oxyresveratrol permeating through shed snake skin from microemulsion at 6 h was 93.04 times compared to that of oxyresveratrol from Vaseline, determined at 20% w/w concentration. In cutaneous HSV-1 infection in mice, oxyresveratrol microemulsion at 20%, 25%, and 30% w/w, topically applied five times daily for 7 days after infection, was significantly effective in delaying the development of skin lesions and protecting from death (p2:1>4:1. The large ME regions were correlated with the ME systems containing cosurfactants. Physicochemical Properties of Microemulsions Based on these pseudoternary phase diagrams, the ORME was selected from the systems using Tween 80/isopropanol with the ratio 1:1 which formed the largest single-phase region compared to other ratios. Several ME formulas were selected from the ME region in the phase diagram for the solubility test, and the formula that could incorporate OR at least 30% w/w with minimum concentration of surfactant and Table I. Composition of Various MEs and Their Oxyresveratrol Solubilizing Capacities
Oil (% w/w)
Surfactant mixture (% w/w)
Water (% w/w)
Maximum OR loading capacity (% w/w)
55 50 40 30 20 20 15 15 15 10 10 10 5 5 5 5
40 45 50 60 70 60 75 65 55 80 70 60 90 80 70 60
5 5 10 10 10 20 10 20 30 10 20 30 5 15 25 35
15 15 15 20 25 20 25 25 20 >30a >30a 20 >30a >30a 25 20
Phase Diagrams ME was prepared using isopropyl myristate as the oil phase, Tween 80 as surfactant, isopropanol as cosurfactant, and water. The pseudoternary phase diagrams of ME systems containing isopropyl myristate; Tween 80; isopropanol at fixed ratios (w/w) of 1:1, 2:1, and 4:1; and water after equilibrium at room temperature were presented in Fig. 1. The shade areas of the pseudoternary phase diagrams referred to the ME regions while the outside (white) areas indicated the turbid regions. All
Data Loading...
