MicroRNA-937 is overexpressed and predicts poor prognosis in patients with colon cancer
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RESEARCH
Open Access
MicroRNA-937 is overexpressed and predicts poor prognosis in patients with colon cancer Huiya Liu1, Lin Ma2, Ling Wang3 and Yizuo Yang4*
Abstract Background: Colon cancer is a heterogeneous tumor and a leading cause of cancer-related mortality. MicroRNA (miRNA) has been proposed as the biomarker in cancers. The aim of this study was to investigate the clinical significance and potential functional role of miR-937 in colon cancer. Methods: In the present study, reverse transcription-quantitative polymerase chain reaction (qRT-PCR) was conducted to examine the expression levels of miR-937 in colon cancer tissues and cell lines. Kaplan-Meier curve and Cox regression analyses were used to determine the prognostic impact of miR-937 on survival. Cell Counting Kit-8 and Transwell assays were performed to examine cell proliferation, migration, and invasion, respectively. Results: miR-937 was significantly upregulated in colon cancer tissues and cell lines. Clinical analysis results showed that miR-937 expression was associated with lymph node metastasis and TNM stage. Patients with high miR-937 expression predicted a shorter overall survival rate. Functionally, overexpression of miR-937 promoted cell proliferation, migration, and invasion, while inhibition of miR-937 inhibited these cellular behaviors in vitro. Conclusions: These results suggested that miR-937 may act as a prognostic biomarker and a potential target for therapeutic strategy, as well as promote proliferation, migration, and invasion of colon cancer. Keywords: miR-937, Colon cancer, Prognosis, Proliferation, Migration, Invasion
Introduction Colon cancer is one of the most common malignant tumors of the digestive tract and the leading cause of cancer-related mortality worldwide [1, 2]. The development of colon cancer is influenced by many factors and is a multi-gene related, multi-step complex process [3]. Colon cancer patients at early stages often have longterm survival, however, colon cancer with early-onset symptoms is not typical and the patients usually initially diagnosed at advanced stages with unsatisfactory prognosis [4]. Despite considerable progress in current therapeutic strategies, such as surgery, chemoradiotherapy, and comprehensive biological treatment, the overall survival rate of advanced colon cancer is still unsatisfactory [5]. Therefore, better cancer-related biomarkers and * Correspondence: [email protected] 4 Department of Geriatrics, Heze Municipal Hospital, No. 2888, Caozhou Road, Heze 274400, Shandong, China Full list of author information is available at the end of the article
therapeutic targets for the treatment of colon cancer are necessary. Numerous studies have demonstrated that abnormal expression of microRNAs (miRNAs) is closely linked to the initiation and development of cancers [6]. MiRNA is a family of endogenous small non-coding RNAs of 19– 24 nucleotides in length and negatively regulate gene expression at the post-transcriptional level by binding to the 3′-UTR of their target mRNA [7, 8].
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