MicroRNA expression profiling and Notch1 and Notch2 expression in minimal deviation adenocarcinoma of uterine cervix
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RESEARCH
WORLD JOURNAL OF SURGICAL ONCOLOGY
Open Access
MicroRNA expression profiling and Notch1 and Notch2 expression in minimal deviation adenocarcinoma of uterine cervix Heejeong Lee1, Kyu Rae Kim2, Nam Hoon Cho3, Sung Ran Hong4, Hoiseon Jeong4, Sun Young Kwon5, Kwang Hwa Park6, Hee Jung An7, Tae Heon Kim7, Insun Kim8, Hye Kyoung Yoon9, Kwang Sun Suh10, Ki Ouk Min1, Hyun Joo Choi1, Ji Young Park11, Chong Woo Yoo12, Youn Soo Lee1, Hee Jin Lee13, Weon Sun Lee13, Chul Soo Park14*, Yonghee Lee15* and The Gynecological Pathology Study Group of the Korean Society of Pathologists
Abstract Background: MicroRNA (miRNA) expression is known to be deregulated in cervical carcinomas. However, no data is available about the miRNA expression pattern for the minimal deviation adenocarcinoma (MDA) of uterine cervix. We sought to detect deregulated miRNAs in MDA in an attempt to find the most dependable miRNA or their combinations to understand their tumorigenesis pathway and to identify diagnostic or prognostic biomarkers. We also investigated the association between those miRNAs and their target genes, especially Notch1 and Notch2. Methods: We evaluated miRNA expression profiles via miRNA microarray and validated them using.real-time PCR assays with 24 formalin-fixed, paraffin-embedded tissue blocks of MDA and 11 normal proliferative endocervical tissues as control. Expression for Notch1 and 2 was assessed by immunohistochemistry. Results: MiRNA-135a-3p, 192-5p, 194-5p, and 494 were up-regulated, whereas miR-34b-5p, 204-5p, 299-5p, 424-5p, and 136-3p were down-regulated in MDA compared with normal proliferative endocervical tissues (all P
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