MicroRNA Expression Detection Methods
This book provides comprehensive descriptions of the innovative strategies and methodologies for detecting miRNA expression, their application in miRNA research and their potential as tools for clinical diagnosis and prognosis. It begins with the introduc
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Zhiguo Wang Baofeng Yang l
MicroRNA Expression Detection Methods
Dr. Zhiguo Wang Montreal Heart Institute Research Center 5000 Belanger Street Montreal QC H1T 1C8 Canada [email protected] or [email protected]
Dr. Baofeng Yang Harbin Medical University Dept. Pharmacology 150086 Harbin China, People’s Republic [email protected]
ISBN: 978-3-642-04927-9 e-ISBN: 978-3-642-04928-6 DOI 10.1007/978-3-642-04928-6 Springer Heidelberg Dordrecht London New York Library of Congress Control Number: PCN Applied for # Springer-Verlag Berlin Heidelberg 2010 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable to prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover design: WMXDesign GmbH, Heidelberg, Germany Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface
MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of 22nucleotides, have rapidly emerged as the central players in gene expression regulation. Owing to their ever-increasing implications in the control of various biological and pathological processes, miRNAs have now been considered novel biomarkers of various human diseases including, cancer, viral disease, cardiovascular disorders, metabolic disturbances, etc. Particular expression profiles have been associated with particular pathological states. Expression profiling of miRNAs have therefore become extremely important not only for fundamentalists but also for clinicians. However, the methodologies used for detecting protein-coding mRNAs cannot be directly applied to miRNAs because of their small size. Over the past years, researchers have made great efforts to developing techniques suitable for miRNA detection and quantification; a wide spectrum of creative and innovative techniques (more than 30 different methods) have been invented and validated. It has come to the time now to summarize these methods and present them in an orderly manner for better understanding and utilization of these methods to miRNA research and applications. In particular, the development of methods for quantifying circulating miRNAs opens up a fascinating opportunity for realizing miRNA as diagnostic and prognostic biomarkers of human disease. A book on this subject may help boosting up the passion of researchers to further improv
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