Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in
- PDF / 1,022,301 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 59 Downloads / 197 Views
GENETICS, EVOLUTION, AND PHYLOGENY - ORIGINAL PAPER
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina Matias A. Dorsch 1,2 Germán J. Cantón 4
Javier Regidor-Cerrillo 3 & Luis M. Ortega-Mora 3 & María V. Scioli 4 & Eleonora L. Morrell 4 & 1,5 4,5 & Dadín P. Moore & Yanina P. Hecker &
Received: 12 August 2020 / Accepted: 5 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa. Keywords Neospora caninum . Bovine . Abortion . Microsatellite markers . Argentina
Introduction Section Editor: Daniel K Howe Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00436-020-06922-x) contains supplementary material, which is available to authorized users. * Yanina P. Hecker [email protected] 1
Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina
2
Animal Health Platform, National Institute of Agricultural Research (INIA), La Estanzuela, Colonia, Uruguay
3
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain
4
National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina
5
Institute of Innovation for Agricultural Production and Sustainable Development (IPADS Balcarce), Balcarce, Argentina
Neosporosis is a globally distributed disease caused by Neospora caninum, a cyst-forming protozoan with a wide host range (Dubey and Schares 2011). Canids are the definitive hosts, while cattle are the most representative intermediate
Data Loading...
