Microsomal reductase activity in patients with thyroid neoplasms
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ORIGINAL ARTICLE
Microsomal reductase activity in patients with thyroid neoplasms Elena V. Proskurnina 1 Maria V. Fedorova Igor V. Panteleev 4 Evgeny V. Svetlov 4 ●
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Madina M. Sozarukova
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Aleksandr E. Mitichkin4
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Received: 26 May 2020 / Accepted: 23 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Objective Cytochrome b5-reductase (CYB5R) and cytochrome P450 reductase (CYPOR) are important for cell metabolism; however, their role in thyroid hormonogenesis and carcinogenesis has not been elucidated yet. The activity of CYB5R correlates with the metastasis in breast cancer, but there are no similar studies for CYB5R and CYPOR for thyroid tumors. The aim of this study was to elucidate the activity of CYB5R and CYPOR changes in benign euthyroid and hyperthyroid neoplasms and in papillary thyroid cancer for their potential application as biomarkers for diagnosis and prognosis prediction of thyroid cancer. Methods Thirty-six patients with thyroid diseases participated in the study. The control euthyroid nodular goiter group included ten patients; the thyrotoxic nodular or diffuse goiter group included 14 patients; the papillary thyroid cancer T12N0-1M0 (PTC) group included 12 patients. The activity of CYB5R and CYPOR was assessed with lucigenin-enhanced chemiluminescence stimulated by NADH and NADPH, respectively. Results Compared to the control euthyroid nodular goiter group, activity of CYB5R and CYPOR increased ~5 and 10 times, respectively, in toxic goiter, and 15 and 30 times, respectively, in half of cases of PTC. The change in activity of CYPOR was more pronounced compared to CYB5R. Within the PTC group, the subgroups with low and high activities of microsomal reductases were identified. Microsomal reductases in follicular adenoma was 2–4-fold less active compared to the euthyroid nodular goiter and the low-activity PTC group. Conclusions Activity of tissue microsomal reductases varies in thyroid pathology and can be considered as a promising biomarker for differential diagnostics of benign and malignant thyroid tumors. Keywords Cytochrome b5-reductase Cytochrome P450 reductase Nontoxic nodular goiter Toxic goiter Papillary thyroid cancer Chemiluminescence ●
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Introduction Thyroid neoplasms are detected in 20–50% of cases of thyroid pathology, 4–5% of cases of which are cancer. Although thyroid cancer is quite rare and makes up from 1
* Elena V. Proskurnina [email protected] 1
Research Centre for Medical Genetics, Moscow, Russia
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Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia
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Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia
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Inozemtsev City Clinical Hospital of Moscow Department of Health, Moscow, Russia
to 4% of all malignant tumors, morbidity of thyroid cancer has increased 2.5 times recently [1]. The development of new approaches to diagnosing and predicting the development of this type of cancer i
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