Microstructured Bioactive Interfaces using Piezoelectric Ink Jet Technology
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0950-D15-23
Microstructured Bioactive Interfaces using Piezoelectric Ink Jet Technology Anand Doraiswamy1, Cerasela Z. Dinu2, Jan Sumerel3, Douglas B. Chrisey2, and Roger J. Narayan1 1 University of North Carolina, Chapel Hill, NC, 27599-7575 2 Rensselaer Polytechnic Institute, Troy, NY, 12180 3 Fujifilm Dimatix, 2230 Martin Avenue, Santa Clara, CA, 95050 Microstructured Bioactive Interfaces using Piezoelectric Ink Jet Technology A. Doraiswamy1, C.Z. Dinu2, D.B. Chrisey2, J. Sumerel3, and R.J. Narayan1 1
University of North Carolina, Chapel Hill, NC, 27599-7575 Rensselaer Polytechnic Institute, Troy, NY, 12180 3 FujiFilm Dimatix, 2230 Martin Avenue, Santa Clara, CA, 95050 2
ABSTRACT We have demonstrated microscale patterning of biotin and streptavidin proteins using an athermal rapid prototyping process based on piezoelectric inkjet technology. A MEMS-based piezoelectric actuator was used to dispense picoliter quantities of fluid through micron-sized nozzles. Atomic force microscopy and Fourier infrared spectroscopy studies were performed on CAD/CAM deposited proteins that were prepared using several firing voltages. Our results indicate that piezoelectric inkjet deposition is a powerful non-contact, non-destructive process for developing highthroughput biological microarrays for use in biosensing, cell culturing, and tissue engineering. INTRODUCTION Microscale patterns of heterogeneous proteins will allow for the development of next generation immunoassays, biosensors, and cell culture devices [1-3]. Twodimensional microscale patterning of biological molecules has been previously achieved using soft lithography [2, 3], elastomeric stenciling [4], microdispensing [5], laser direct writing [6], matrix-assisted pulsed laser evaporation [7], and dip pen nanolithography [8]. There is a need for a rapid prototyping technique for preparing high throughput microscale patterns of biological molecules without the use of masks, stamps, or ribbons. One such technique is a non-contact piezoelectric ink jetting. This process involves the creation of a rapidly moving stream of fluid that passes through a small nozzle. In thermal non-contact printing, drops are created by thermal stimulus [9, 10]. A resistive element is used to heat the fluid, creating a bubble that forces the ink out of the nozzle. A similar process has been utilized for printing proteins and cells [11-13]. However, the thermal stimulus does not entirely preclude damage to biological materials. Syringe-solenoid- and piezoelectric-based athermal ink jet printing systems have also been developed for microscale patterning of materials [14]. In a solenoid system, a syringe pump is used with a microsolenoid actuator to create controlled fluid flow across
an orifice. However, high internal pressures can result in damage to the print head. In a piezoelectric inkjet system, piezoelectric crystals are used to create mechanical vibrations to control fluid flow through the nozzle [15]. Resolution of the printed features is dependent on several factors, including fluid
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