miR169 and PmRGL2 synergistically regulate the NF-Y complex to activate dormancy release in Japanese apricot ( Prunus mu
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miR169 and PmRGL2 synergistically regulate the NF-Y complex to activate dormancy release in Japanese apricot (Prunus mume Sieb. et Zucc.) Jie Gao1 · Xiaopeng Ni1 · Hantao Li1 · Faisal Hayat1 · Ting Shi1 · Zhihong Gao1 Received: 25 March 2020 / Revised: 28 August 2020 / Accepted: 6 September 2020 © Springer Nature B.V. 2020
Abstract Key message This study is the first to demonstrate that G A4-induced dormancy release is associated with the NF-Y complex, which interacts with gibberellin inhibitor RGL2 in Japanese apricot. Abstract Seasonal dormancy is not only vital for the survival in cold winter but also affects flowering of temperate fruit trees and the dormancy release depends on the accumulation of the cold temperatures (Chilling requirement-CR). To understand the mechanism of dormancy release in deciduous fruit crops, we compared miRNA sequencing data during the transition stage from paradormancy to dormancy release in the Japanese apricot and found that the miR169 family showed significant differentially up-regulated expression during dormancy induction and was down-regulated during the dormancy release periods. The 5′ RACE assay and RT-qPCR validated its target gene NUCLEAR FACTOR-Y subunit A (NF-YA), which exhibited the opposite expression pattern. Further study showed that exogenous G A4 could inhibit the expression of the gibberellic acid (GA) signal transduction suppressor PmRGL2 (RGA-LIKE 2) and promote the expression of NF-Y. Moreover, the interaction between the NF-Y family and GA inhibitor PmRGL2 was verified by the yeast-two-hybrid (Y2H) system and a bimolecular fluorescence complementarity (BiFC) experiment. These results suggest that synergistic regulation of the NF-Y and PmRGL2 complex leads to the activation of dormancy release induced by G A4. These findings will help to elucidate the functional and regulatory roles of miR169 and NF-Y complex in seasonal bud dormancy induced by GA in Japanese apricot and provide new insights for the discovery of dormancy release mechanisms in woody plants. Keywords Japanese apricot · MicroRNAs · PmNF-YA3 · GA4 · 5′ RLM-RACE · Flower bud dormancy
Introduction In winter, perennial woody trees, including deciduous fruit trees, have to face severe threats, like low temperature and a short photoperiod in temperate zones. Flower bud dormancy is a bet-hedging strategy to prevent injury in inappropriate circumstances and synchronise perennial growth (Zhang et al. 2018b). Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01070-3) contains supplementary material, which is available to authorized users. * Zhihong Gao [email protected] 1
Laboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Bud dormancy influences the flowering time and the quality of flower buds, which could affect the yield and quality of fruit in fruit crops (Campoy et al. 2011; Footitt et al. 2014). Lang et al. (1987) divided the pattern of dormancy into thre
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