Molecular and genome characterization of colistin-resistant Escherichia coli isolates from wild sea lions ( Zalophus cal
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ENVIRONMENTAL MICROBIOLOGY - SHORT COMMUNICATION
Molecular and genome characterization of colistin-resistant Escherichia coli isolates from wild sea lions (Zalophus californianus) Rigoberto Hernández-Castro 1 & Jonathan Rodríguez-Santiago 2 & Juan Téllez-Sosa 3 & Sugey Bravo-Romero 3 & Jesús Silva-Sánchez 2 & Alejandro Sánchez-Pérez 2 & Rosalia Avalos-Téllez 4 & Luary Carolina Martínez-Chavarría 5 & Juan Xicohtencatl-Cortes 6 & Ulises Garza-Ramos 2 Received: 7 August 2020 / Accepted: 26 August 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract Using molecular and whole-genome sequencing tools, we investigated colistin-resistant Escherichia coli isolates from wild sea lions. Two unrelated E. coli colistin-resistant isolates, ST8259 and ST4218, were identified, both belonging to the B2 phylogroup and different serotypes. Polymorphisms in PmrA, PmrB, and PhoQ proteins were identified, and the role of PmrB and PhoQ in contributing to colistin resistance was determined by complementation assays. However, the mutations characterized in the present study are not involved in colistin resistance, which have been described in E. coli isolates from clinical settings. Therefore, the acquired mutations in pmrB and phoQ genes in resistance to colistin in bacteria related to marine environment animals are different. This work contributes to the surveillance and characterization of colistin resistance in Escherichia coli obtained from animals from aquatic environments. Keywords Aquatic environments . Antimicrobial resistance . Chromosomal mutations . Two-component system
Introduction The rapid emergence and spread of multidrug-resistant Gramnegative bacteria have renewed interest in colistin as the last resort treatment [1]. The multiple chromosomal mutations
associated with acquired colistin resistance are concerning [2]. Later, the mcr-1 plasmid-mediated colistin resistance gene was identified in China [3] and then quickly identified on all continents and in a wide variety of ecological niches [4, 5]. There are eight known mechanisms of colistin resistance
Rigoberto Hernández-Castro and Jonathan Rodríguez-Santiago contributed equally to this work. Responsible Editor: Nilton Lincopan. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42770-020-00371-5) contains supplementary material, which is available to authorized users. * Ulises Garza-Ramos [email protected] 1
Departamento Ecología de Agentes Patógenos, Hospital General “Dr. Manuel Gea González”, Ciudad de México, Mexico
2
Laboratorio de Resistencia Bacteriana; Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Instituto Nacional de Salud Pública (INSP), Cuernavaca, Morelos, Mexico
3
Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Instituto Nacional de Salud Pública (INSP), Cuernavaca, Morelos, Mexico
4
Área de Protección de Flora y Fauna. Islas del Golfo de California, Baja California, CONANP, SEMARNAT. Bahía de Los Ángeles, 22980 Ensenada, Baja California, Mexico
5
Departament
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