Antimicrobial resistance and virulence genes in the Escherichia coli isolates obtained from ostrich
- PDF / 296,408 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 97 Downloads / 250 Views
REGULAR ARTICLES
Antimicrobial resistance and virulence genes in the Escherichia coli isolates obtained from ostrich Fatemeh Amani 1 & Gholamreza Hashemitabar 1 & Abolfazl Ghaniei 2
&
Hamidreza Farzin 3
Received: 15 March 2020 / Accepted: 10 September 2020 # Springer Nature B.V. 2020
Abstract Escherichia coli (E.coli) is a normal intestinal microflora of birds, including ostrich. However, some strains are pathogenic to ostrich. There is a lack of knowledge concerning the differences between commensal and pathogenic E.coli isolates of ostrich. This research aimed to characterize the antimicrobial susceptibility profile and virulence gene content of presumptive commensal and pathogenic E.coli isolates of ostrich. A total of 129 bacterial isolates (commensal strains no. = 45; pathogenic strains no. = 84) were obtained from ostriches. The resistance profile of these isolates was investigated by the disk diffusion method and PCR. Also, the strains were screened for virulence-associated genes of avian pathogenic E.coli (APEC). The study showed that the highest and the lowest antimicrobial resistance were against oxytetracycline and gentamicin, respectively. Oxytetracycline, florfenicol, and streptomycin resistance rate in pathogenic isolates were higher than commensal ones (p < 0.05). Also, tetA, blaTEM, and aac(3)-IV resistance genes were more prevalent in pathogenic than commensal isolates (p < 0.05). More than half of the isolates had no virulence-associated genes. The multiplex PCR results showed that irp2 gene was more prevalent in pathogenic than commensal E.coli (p < 0.05). Nevertheless this was not the case with the other genes. Our results indicated a low frequency of antimicrobial resistance and virulence genes in E.coli isolates of ostriches. Antimicrobial susceptibility profile and virulence gene content of E.coli isolates of ostriches differ between presumptive commensal and pathogenic strains. However, more analyses are needed to discriminate these isolates. Keywords Pathogenic E.coli . Commensal E.coli . Antimicrobial susceptibility . Virulence genes . Ostrich
Abbreviations E.coli Escherichia coli APEC Avian Pathogenic Escherichia coli.
Introduction During the 1990s, Iran imported ostrich from abroad. The ostrich farming industry has developed a lot in these years * Abolfazl Ghaniei [email protected] 1
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
3
Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran
because of the rising demand for its valuable products (Rezaei Far et al., 2013). However, this industry needs more improvement in the production efficiency of ostrich herds. Some factors such as bacterial pathogens have a considerable effect on the profitability of the flocks. E.coli causes yolk sac infection during fetal and neonatal life
Data Loading...
