Molecular-based cross-species evaluation of bovine coronavirus infection in cattle, sheep and goats in Ghana
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RESEARCH ARTICLE
Open Access
Molecular-based cross-species evaluation of bovine coronavirus infection in cattle, sheep and goats in Ghana Vitus Burimuah1,2,3*, Augustina Sylverken3,4, Michael Owusu3,5, Philip El-Duah1,3,6, Richmond Yeboah1,3, Jones Lamptey1,3, Yaw Oppong Frimpong3,7, Olivia Agbenyega8, Raphael Folitse2, Ben Emikpe2, William Tasiame2,6, Eddie-Williams Owiredu9, Samuel Oppong10, Christopher Antwi7, Yaw Adu-Sarkodie1 and Christian Drosten6
Abstract Background: Apart from the huge worldwide economic losses often occasioned by bovine coronavirus (BCoV) to the livestock industry, particularly with respect to cattle rearing, continuous surveillance of the virus in cattle and small ruminants is essential in monitoring variations in the virus that could enhance host switching. In this study, we collected rectal swabs from a total of 1,498 cattle, sheep and goats. BCoV detection was based on reverse transcriptase polymerase chain reaction. Sanger sequencing of the partial RNA-dependent RNA polymerase (RdRp) region for postive samples were done and nucleotide sequences were compared with homologous sequences from the GenBank. Results: The study reports a BCoV prevalence of 0.3%, consisting of 4 positive cases; 3 goats and 1 cattle. Less than 10% of all the animals sampled showed clinical signs such as diarrhea and respiratory distress except for high temperature which occurred in > 1000 of the animals. However, none of the 4 BCoV positive animals manifested any clinical signs of the infection at the time of sample collection. Bayesian majority-rule cladogram comparing partial and full length BCoV RdRp genes obtained in the study to data from the GenBank revealed that the sequences obtained from this study formed one large monophyletic group with those from different species and countries. The goat sequences were similar to each other and clustered within the same clade. No major variations were thus observed between our isolates and those from elsewhere. (Continued on next page)
* Correspondence: [email protected] 1 Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana 2 School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need
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