Molecular characteristics of the capsid protein VP2 gene of canine parvovirus type 2 amplified from raccoon dogs in Hebe
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ORIGINAL ARTICLE
Molecular characteristics of the capsid protein VP2 gene of canine parvovirus type 2 amplified from raccoon dogs in Hebei province, China Rongguang Lu1,2 · Yongle Yu2 · Xiangyu Zhu1 · Qiumei Shi3 · Yang Wang1 · Jigui Wang2 · Shuang Lv1 · Ning Shi1 · Hao Liu4 · Xiaoyu Deng1 · Shizhen Lian1 · Minghao Yan5 · Hang Zhao6 · Bo Hu1 · Weiquan Liu2 · Xijun Yan1 Received: 21 December 2019 / Accepted: 22 May 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Canine parvovirus type 2 (CPV-2) is currently circulating in domestic and wild animals, but our knowledge about CPV-2 infections in raccoon dogs is limited. In this study, VP2 gene sequences of CPV-2 were amplified from rectal swabs of 14 diarrhetic raccoon dogs (Nyctereutes procyonoides) in Hebei province, China, in 2016 and 2017. Phylogenetic analysis of the VP2 gene sequences revealed that most of these sequences (11 of 14) belonged to the same subclade as raccoon dog strain CPV-2/Raccoon_Dog/China/DP-1/16 isolated from Shandong province in 2016. A comparison of deduced amino acid sequences revealed presence of the substitutions S297A and S27T in 11 of those 14 sequences. I418T was observed in a minority of the sequences (4 of 14). In addition, A300D and T301I, P13S and I219V, and N419K were found in three of the sequences. This study shows that CPV-2 strains with different substitutions in their VP2 amino acid sequences were spreading among raccoon dogs in Hebei during 2016 and 2017 and suggests that further studies are needed to monitor the distribution of these strains in China.
Introduction
Handling Editor: Ana Cristina Bratanich. Rongguang Lu, Yongle Yu, and Xiangyu Zhu contributed equally to this work and should be regarded as co-first authors. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04714-3) contains supplementary material, which is available to authorized users. * Weiquan Liu [email protected] * Xijun Yan [email protected] 1
2
Key Laboratory of Special Animal Epidemic Disease of Ministry of Agriculture and Rural Affairs, Institute of Special Animals and Plants Sciences, Chinese Academy of Agricultural Sciences, No. 4899 Juye Street, Changchun 130112, Jilin, China State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China
Since the late 1970s, canine parvovirus type 2 (CPV-2) has been recognized as a new etiological agent causing enteritis in dogs (Canis lupus familiaris) and wild carnivores, and it has been spreading worldwide for a few years [5, 15]. It was named CPV-2 to distinguish it from the previously described parvovirus of dogs, minute virus of canines (MVC or CPV1) [13]. CPV-2 belongs to the species Carnivore protoparvovirus 1 (genus Protoparvovirus, family Parvoviridae), 3
Key Laboratory of Preventive Veterinary Medicine in Hebei Province, Hebei Normal University of Science &
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