Molecular characterization of Cryptosporidium spp. in minks ( Neovison vison ), blue foxes ( Vulpes lagopus ), and racco
- PDF / 423,200 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 27 Downloads / 179 Views
PROTOZOOLOGY - SHORT COMMUNICATION
Molecular characterization of Cryptosporidium spp. in minks (Neovison vison), blue foxes (Vulpes lagopus), and raccoon dogs (Nyctereutes procyonoides) in farms from Xinjiang, Northwest China Weifeng Qian 1,2 & Ying Zhang 2 & Yuxi Jiang 2 & Aiyun Zhao 2 & Chaochao Lv 1 & Meng Qi 2 Received: 3 August 2019 / Accepted: 27 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The objective of this study was to determine the infection rate and genetic diversity of Cryptosporidium spp. in minks, foxes, and raccoon dogs, farmed in the Xinjiang Uygur Autonomous Region, Northwest China. Fresh fecal specimens were collected from individual cages of farmed minks (n = 214), blue foxes (n = 35), and raccoon dogs (n = 39) and examined using nested PCR based on the Cryptosporidium spp. small subunit rRNA gene. Cryptosporidium spp. was detected in 35 cages (12.2%, 35/288), with a higher infection rate detected in raccoon dogs (20.5%) compared with minks (12.1%) and blue foxes (2.9%). Sequence analysis showed that Cryptosporidium canis was the only species identified in blue foxes and raccoon dogs, while in the 26 Cryptosporidium-positive mink specimens, Cryptosporidium mink genotype (n = 17), C. canis (n = 7), and Cryptosporidium parvum (n = 2) were identified. Further analysis based on the 60-kDa glycoprotein (gp60) gene determined that both C. parvum isolates belonged to the subtype IIdA15G1, while eight of the 17 Cryptosporidium mink genotype isolates were a novel subtype that we have named XeA5G1. To the best of our knowledge, this is the first report of C. parvum subtype IIdA15G1 infection in minks. Since all the Cryptosporidium species/genotypes identified in minks, foxes, and raccoon dogs from Xinjiang have been previously found in humans, our results suggest that these fur animals may play a role in the transmission of zoonotic Cryptosporidium. Keywords Cryptosporidium . Zoonotic . Subtyping . Mink . Fox . Raccoon dog . China
Introduction Minks (Neovison vison), blue foxes (Vulpes lagopus), and raccoon dogs (Nyctereutes procyonoides) are some of the major animals farmed in China, and their furs and products have a high economic value, and in 2017, the farmed populations of these animals reached roughly 20 million heads (http://org. caaa.cn/article.php?id=15180). It has been reported that parasites negatively influence the health status of fur animals causing important losses to farms (Yang et al. 2018). In this Section Editor: Yaoyu Feng * Meng Qi [email protected] 1
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China
2
College of Animal Science, Tarim University, Tarim Road 1487, Alar 843300, Xinjiang, China
context, Cryptosporidium spp. are important protozoan that can also infect humans and a wide range of other vertebrates, mainly causing intestinal disease (Fayer 2010). Global data showed that Cryptosporidium prevalence in farmed minks, blue foxes, and raccoon dogs could be as high as 38.7% (Y
Data Loading...
