Molecular characterization of water extractable Euglena gracilis cellular material composition using asymmetrical flow f
- PDF / 589,645 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 11 Downloads / 165 Views
RESEARCH PAPER
Molecular characterization of water extractable Euglena gracilis cellular material composition using asymmetrical flow field-flow fractionation and high-resolution mass spectrometry Ainsely Lewis 1 & Céline Guéguen 2 Received: 15 February 2020 / Revised: 16 March 2020 / Accepted: 7 April 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Asymmetrical flow field-flow fractionation (AF4) and high-resolution Orbitrap mass spectrometry (HRMS) were used to separate and characterize cellular fractions of the dark- and light-grown Euglena gracilis cellular material. Biological replicates analyzed by HRMS shared 21–73% of commonly detected m/z values. Greater variability in shared features was found in lightgrown cellular fractions (p < 0.05), likely due to small variations in growth stage. Significant differences in molecular composition were observed between AF4 cellular fractions, with dark cell fractions showing a propensity towards carbohydrate-like and tannin-like compounds, and higher double-bond equivalent (DBE) and modified aromatic index (AImod) were associated with light-grown cell fractions. Fractionation and high-resolution mass spectrometry aided characterization demonstrated the power of the AF4 to selectively cater to certain compounds/cellular entities with distinct compositional classes and double-bond equivalents and aromaticity index characteristics. Keywords Microalgae . Fractionation . Molecular characterization . Euglena . Orbitrap
Introduction Algae account for more than half the total primary production of the food chain worldwide [1] and can have significant impact on the regional and global carbon cycle [2]. The algal species and its growing conditions can influence the types of compounds produced intracellularly [3–5]. To better understand algal biochemical composition, various characterization techniques are often used such as excitation emission matrix spectroscopy (EEM; [6]), Fourier-transform infrared spectroscopy (FTIR; [7]), nuclear magnetic resonance spectroscopy (NMR; [8]), and X-ray photoelectron spectroscopy (XPS; [9]). Although these methods can characterize biochemical Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02650-4) contains supplementary material, which is available to authorized users. * Céline Guéguen [email protected] 1
Environmental and Life Sciences Graduate Program, Trent University, Peterborough, Ontario K9J 7B8, Canada
2
Département de chimie, Université de Sherbrooke, 2500 Boulevard de l’université, Sherbrooke, Québec J1K 2R1, Canada
aspects of algae, they are not sensitive for investigating molecular level chemical properties. With the recent advancements of high-resolution mass spectrometry (HRMS), identifying thousands of molecular structures with high accuracy (sub-ppm) is possible [10–14] with minimal ambiguity of formula assignments [15]. The untargeted HRMS approach is favored when no extended molecular knowledge exists [16], as it can reveal ne
Data Loading...
