Cloning and expression profiling of muscle regulator ANKRD2 in domestic chicken Gallus gallus
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ORIGINAL PAPER
Cloning and expression profiling of muscle regulator ANKRD2 in domestic chicken Gallus gallus Nemanja Stamenkovic1 · Jovana Jasnic1 · Mirjana Novkovic1 · Emilija Milosevic1 · Srdjan Boskovic1 · Ana Kojic1,2 · Kristina Popic1,3 · Marija Stankovic1 · Yajun Wang4 · Sanja Milenkovic5 · Dragica Radojkovic1 · Guoda Ma6 · Snezana Kojic1 Accepted: 2 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Striated muscle signaling protein and transcriptional regulator ANKRD2 participates in myogenesis, myogenic differentiation, muscle adaptation and stress response. It is preferentially expressed in slow, oxidative fibers of mammalian skeletal muscle. In this study, we report on characterization of chicken ANKRD2. The chicken ANKRD2 coding region contains 1002 bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of the ANKRD2 gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to its selective expression in slow, oxidative, type I and fast, oxidative–glycolytic, type IIA myofibers. Association of chicken ANKRD2 with phenotypic differences between red and white muscles points to its potential role in the process of myofiber-type specification. In addition to expression in slow oxidative myofibers, as demonstrated for mammalian protein, chicken ANKRD2 was also detected in fast fibers with mixed oxidative and glycolytic metabolism. This finding suggests that ANKRD2 is responsive to metabolic differences between types of avian myofibers and orientates future studies towards investigation of its role in molecular mechanisms of myofiber-type-specific gene expression. Keywords ANKRD2 · Chicken · Slow muscle fiber · Oxidative metabolism · Skeletal muscle
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00418-020-01899-1) contains supplementary material, which is available to authorized users. * Snezana Kojic [email protected] 1
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
2
European Molecular Biology Laboratory (EMBL), Heidelberg University, Heidelberg, Germany
3
VINCA Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia
4
Clinical Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China
5
Clinical Hospital Center Zemun, Belgrade, Serbia
6
Maternal and Child Institute, Shunde Women and Children’s Hospital, Guangdong Medical University, Foshan, China
ANKRD2 (ankyrin repeats containing domain 2) or Arpp (ankyrin repeat, PEST sequence, and prol
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