Molecular marker aided selection for developing rust resistant genotypes by pyramiding Lr19 / Sr25 and Yr15 in wheat ( T
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ORIGINAL PAPER
Molecular marker aided selection for developing rust resistant genotypes by pyramiding Lr19/Sr25 and Yr15 in wheat (Triticum aestivum L.) D. Pal 1 & S. C. Bhardwaj 2 & P. Sharma 1 & D. Sharma 2 & H. Khan 3 & Harikrishna 4 & H. P. Babu 4 & S. K. Jha 4 & M. Patial 1 & D. Chauhan 4 & S. Kumari 4 & K. V. Prabhu 5 Received: 24 December 2019 / Accepted: 24 August 2020 # Australasian Plant Pathology Society Inc. 2020
Abstract The study was aimed to develop rust resistant genotypes with genome of prominent wheat variety HS240 by pyramiding of linked leaf rust and stem rust resistance genes Lr19 and Sr25 with stripe rust resistance gene Yr15. The genotypes FLW13 and FLW20 were used as gene donors for transfer of Yr15 and Lr19/Sr25, respectively. The molecular markers scs265, wmc221, PSY1-E1, and Gb linked to Lr19/Sr25 and barc8 and gwm11 linked to Yr15 were used for foreground selection. Background selection, involving 58 simple sequence repeat (SSR) markers polymorphic between HS240 and FLW20; 72 between HS240 and FLW13 was carried out to recover the genome of HS240 in advanced bulks derived from a cross HS240*2/FLW20//HS240*2/FLW13. The SSR based genome recovery in selected advanced breeding lines, WBM3682 and WBM3684 was 90.8% and 93.3%, respectively. The 35 K SNP array based analysis of genomic regions of HS240 also substantiated the results of genomic recovery estimated through SSR markers. The selected line WBM3682 recorded average grain yield of 25.7q/ha and showed its superiority over parental check HS240 under rainfed situations. This study has led to development of rust resistant wheat carrying genome of HS240. Keywords Wheat . Rust resistance . Pyramiding . Lr19/Sr25 . Yr15 Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13313-020-00738-0) contains supplementary material, which is available to authorized users. * D. Pal [email protected]
D. Chauhan [email protected]
S. C. Bhardwaj [email protected]
S. Kumari [email protected]
P. Sharma [email protected]
K. V. Prabhu [email protected]
D. Sharma [email protected]
1
Regional Station (Cereals and Horticultural Crops), Tutikandi Facility, ICAR-Indian Agricultural Research Institute, Shimla, Himachal Pradesh 171004, India
2
ICAR-IIWBR, Regional Station, Flowerdale, Shimla, Himachal Pradesh 171002, India
3
ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana 132001, India
4
ICAR- Indian Agricultural Research Institute, New Delhi 110012, India
5
Protection of Plant Varieties and Farmers’ Right Authority, New Delhi 110012, India
H. Khan [email protected] Harikrishna [email protected] H. P. Babu [email protected] S. K. Jha [email protected] M. Patial [email protected]
D. Pal et al.
Introduction The wheat crop cultivated in 215 million hectares every year (wheat.org/wheat-in-the-world/) is affected by several biotic stresses. Among wheat diseases, rusts are the foremost and cause huge loss with a po
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