Monolithic Column Prepared with UiO-66-2COOH MOF as Monomer for Enrichment and Purification of Ursolic Acid in Plants by
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ORIGINAL
Monolithic Column Prepared with UiO‑66‑2COOH MOF as Monomer for Enrichment and Purification of Ursolic Acid in Plants by Online Solid‑Phase Extraction Xiaoya Pang1,2 · Haiyan Liu1,2 · Huan Yu1,2 · Miaomiao Zhang1,2 · Ligai Bai1,2 · Hongyuan Yan1,2 Received: 8 January 2020 / Revised: 24 June 2020 / Accepted: 30 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A method for preparing a metal–organic framework (MOF)–polymeric monolith was established by in situ radical polymerization with derivatized UiO-66-2COOH and N-isopropylacrylamide (NIPAAm) as co-monomers. It was worth noting that the obtained monolith possessed characteristics of relatively uniform porous structure and a high surface area (84.16 m2 g−1). The adsorption capacity of the monolith increased from 22.87 to 44.92 mg−1 due to the addition of MOF. The monolith was used as adsorbent and coupled with HPLC for the online purification and extraction of ursolic acid (UA) in medicinal plant. The limit of detection (LOD, S/N = 3) and the limit of quantification (LOQ, S/N = 10) were 0.17 μg m L−1 and 0.57 μg m L−1, respectively; the relative standard deviation (RSD) of intra-day and inter-day assays for five plant samples were 1.00–3.67% and 2.43–5.76%, respectively; a good accuracy was demonstrated by the recovery of 92.2–103.28%. The results indicated that the MOF–polymer monolith was feasible for the online enrichment and purification of UA in plant. Keywords MOF–polymer monolith · Online solid-phase extraction · Ursolic acid · Purification · Enrichment
Introduction As a natural pentacyclic triterpenic acid compound, UA widely exists in nature [1]. It has received wide attention for its therapeutic activities [2]. The research showed that UA had many pharmacological activities, including antiinflammatory actions, antioxidant effects, anticarcinogenic effects, and antitumor et al. [3‒7].
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10337-020-03931-x) contains supplementary material, which is available to authorized users. * Haiyan Liu [email protected] * Hongyuan Yan [email protected] 1
Key Laboratory of Public Health Safety of Hebei Province, College of Pharmacy, Hebei University, Baoding 071002, China
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China
2
Herbal plant samples have a complex matrix and contain various biologically active ingredients, which leads to serious interference in the analysis of specific bioactive components. Hence, it is especially important to establish an accurate and simple analytical method for these compounds. Some methods have developed for the pretreatment of UA, such as liquid–liquid extraction (LLE) [8], microwaveassisted extraction (MAE) [9], ultrasound-assisted extraction (UAE) [10], accelerated solvent extraction (ASE) [11], supercritical fluid extraction (SFE) [12], and solid-phase e
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