Morphological and COI Sequence Based Characterisation of Marine Polychaete Species from Great Nicobar Island, India
DNA barcoding has proved to be a powerful alternative method to traditional morphological approaches, allowing to complement identification techniques for living organisms. In this study we assess intraspecific and interspecific genetic divergence Among t
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Abstract DNA barcoding has proved to be a powerful alternative method to traditional morphological approaches, allowing to complement identification techniques for living organisms. In this study we assess intraspecific and interspecific genetic divergence Among the 6 genera marine polychaetes from Great Nicobar Island of Souther part of the Andaman and Nicobar Island. The present study results suggested that high level of interspecific genetic variation was observed between Lysidice collaris and Terebella ehrenbergi (0.727). The minimum genetic distance (0.316) was observed between genera Phyllodoce fristedti and Ceratonereis mirabilis. Morphological identification of the polychaetes in this study was supported by the molecular data, as shown by the congruence and high similarity between the sequences produced in the present study and those available in GenBank. This study presents the first information on DNA barcoding for polychaetes species in the Great Nicobar Island, and it establishes the effectiveness of DNA barcoding for identification of marine polychaetes species from Andaman and Nicobar Island, thus making it available to a much broader range of scientists.
Keywords Polychaetes Cytochrome c oxidase subunit I Great Nicobar Island Andaman and Nicobar Island
V. Sekar (&) R. Rajasekaran C. Prasannakumar R. Sankar Faculty of Marine Sciences, Centre of Advanced in Marine Biology, Annamalai University, Parangipettai Campus, Chidambaram 608502, Tamil Nadu, India e-mail: [email protected] V. Sachithanandam Department of Ocean Studies and Marine Biology, Pondicherry University, Andaman and Nicobar Island 744112, India e-mail: [email protected] Present Address: V. Sekar R. Sankar R. Sridhar V. Sachithanandam National Centre for Sustainable Coastal Management, Ministry of Environment, Forest and Climate Change, Chennai 600025, India © Springer International Publishing Switzerland 2016 S. Trivedi et al. (eds.), DNA Barcoding in Marine Perspectives, DOI 10.1007/978-3-319-41840-7_6
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1 Introduction The world is facing a global biodiversity crisis (Novacek and Cleland 2001; Bellwood et al. 2004). The rapid loss of marine and terrestrial biodiversity has prompted efforts to catalogue this biodiversity in Census of Marine Life (CoML). Nowadays morphologically taxonomical identifications are declining in number the reason behind this being the decline of taxonomic skills (Hopkins and Freckleton 2002) and insufficient funding. Besides, the upcoming scientists focus on the advance techniques of molecular methods. Annelida, one of the most successful animal phyla, exhibits an amazing variety of morphological forms. Disparity between some of the forms is so great that until molecular tools were used. Some annelid lineages were not commonly recognized as belonging to the group (Halanych and Janosik 2006). Generally polychaetes are the most dominant and diverse macrofaunal groups in marine benthic habitats. Usually they characterized up to 80 percentages of high species richnes
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