Morphological and genetic characterisation of Centrorhynchus clitorideus (Meyer, 1931) (Acanthocephala: Centrorhynchidae
- PDF / 1,694,710 Bytes
- 12 Pages / 547.087 x 737.008 pts Page_size
- 110 Downloads / 178 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
Morphological and genetic characterisation of Centrorhynchus clitorideus (Meyer, 1931) (Acanthocephala: Centrorhynchidae) from the little owl Athene noctua (Scopoli) (Strigiformes: Strigidae) in Pakistan Qing Zhao . Nehaz Muhammad . Hui-Xia Chen . Jun Ma . Suleman . Liang Li
Received: 13 January 2020 / Accepted: 25 July 2020 Ó Springer Nature B.V. 2020
Abstract Centrorhynchus Lu¨he, 1911 is a large genus of acanthocephalans mainly parasitic in various strigiform and falconiform birds. Some species of Centrorhynchus have not been adequately described. Here, the detailed morphology of C. clitorideus (Meyer, 1931) was studied using light and, for the first time, scanning electron microscopy, based on newly collected specimens from the little owl Athene noctua (Scopoli) (Strigiformes: Strigidae) in Pakistan. Partial sequences of the 18S and 28S nuclear ribosomal RNA genes and the mitochondrial cytochrome c oxidase subunit 1 (cox1) of C. clitorideus were generated for the first time. No nucleotide variation was detected for the partial 18S and 28S regions, but 3.30% of intraspecific nucleotide divergence was found for the cox1 gene. Phylogenetic analyses based on 28S and 18S sequence data showed that C.
This article is part of the Topical Collection Acanthocephala. Q. Zhao H.-X. Chen L. Li (&) Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei, People’s Republic of China e-mail: [email protected] N. Muhammad J. Ma Suleman State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, People’s Republic of China
clitorideus formed a sister relationship with Centrorhynchus sp. MGV-2005 or Centrorhynchus sp. MGV-2005 ? C. microcephalus (Bravo-Hollis, 1947), respectively.
Introduction The genus Centrorhynchus Lu¨he, 1911 (Acanthocephala: Centrorhynchidae) includes approximately 100 nominal species, which are commonly parasitic in various strigiform and falconiform birds (Amin, 2013; Lisitsyna & Greben, 2015; Smales et al., 2018; Steinauer et al., 2019). It is difficult to identify and differentiate some species of Centrorhynchus based on the morphological characters alone, because of their extraordinary morphological similarity and/or the poor original descriptions. Moreover, the presence of broad ranges of morphological variability in different groups of acanthocephalans has been increasingly reported (Amin & Redlin, 1980; Shostak et al., 1986; Sobecka et al., 2012; Li et al., 2017, 2019). It is not easy to evaluate the broad ranges of morphological variability belonging to intraspecific or interspecific variation based only on traditional taxonomic methods. Some recent studies support the use of nuclear and mitochondrial DNA sequences as genetic markers [i.e. small ribosomal DNA (18S) an
Data Loading...
