Morphological and genetic characterization of Eimeria chalcoptereae n. sp. (Apicomplexa: Eimeriidae) in a common bronzew
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PROTOZOOLOGY - ORIGINAL PAPER
Morphological and genetic characterization of Eimeria chalcoptereae n. sp. (Apicomplexa: Eimeriidae) in a common bronzewing pigeon (Phaps chalcoptera) (Latham, 1790) in Western Australia Rongchang Yang 1,2
&
Belinda Brice 3 & Bruno P Berto 4 & Una M Ryan 1
Received: 11 June 2020 / Accepted: 4 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A new Eimeria species is described from a common bronzewing pigeon (Phaps chalcoptera) (Latham, 1790) in Western Australia. Sporulated oocysts of Eimeria chalcoptereae n. sp. (n = 30) are subspheroidal, 22–25 × 21–24 (23.5 × 22.6) μm; length/width (L/W) ratio 1.0–1.1 (1.04) μm. Wall bi-layered, 1.0–1.4 (1.2) μm thick, outer layer smooth, c.2/3 of total thickness. Micropyle barely discernible. Oocyst residuum is absent, but 2 to 3 small polar granules are present. Sporocysts (n = 30) ellipsoidal, 13–14 × 7–8 (13.5 × 7.2) μm; L/W ratio 1.8–2.0 (1.88). Stieda body present, flattened to half-moon-shaped, 0.5 × 2.0 μm; sub-Stieda present, rounded to trapezoidal, 1.5 × 2.5 μm; para-Stieda body absent; sporocyst residuum present, usually as an irregular body consisting of numerous small granules that appear to be membrane-bound. Sporozoites vermiform, with a robust refractile body and centrally located nucleus. Isolated Eimeria oocysts were analysed at the 18S and 28S ribosomal RNA and the mitochondrial cytochrome oxidase (COI) loci. Analyses revealed that Eimeria chalcoptereae n. sp. shared the highest number of molecular features with an Eimeria sp. previously identified from a domestic pigeon in Australia (KT305927-29), with similarities at these three loci of 98.53%, 97.32% and 94.93%, respectively. According to morphological and molecular analysis, the isolated coccidian parasite is a new species of Eimeria named Eimeria chalcoptereae n. sp. after its host, the common bronzewing pigeon (Phaps chalcoptera) (Columbiformes: Columbidae) (Latham, 1790). Keywords Eimeria . Eimeriidae . Common bronzewing pigeon . Morphology . 18S rRNA . 28S rRNA . COI loci
Introduction
Handling Editor: Julia Walochnik * Rongchang Yang [email protected] 1
College of Science, Health, Engineering and Education, Murdoch University, Murdoch, Western Australia 6150, Australia
2
Australian National Phenome Centre, Health Futures Institute, Murdoch University, Harry Perkins Building, Western Australia 6150 Perth, Australia
3
Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia 6076, Australia
4
Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, BR-465 km 7, Seropédica, RJ 23897-000, Brazil
Coccidia are a diverse, complex group of protozoan parasites which infect a wide variety of avian and mammalian species (Tenter et al. 2002). Coccidiosis is an important disease which is a result of infection with these protozoan parasites that belong to the genus Eimeria (Coccidia: Eimeriidae). More than 1700 Eimeria species have been ide
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