Morphological analysis of Spirulina sp. NCIM 5143 and Nostoc ellipsosporum NCIM 2786 and comparative characterization of
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ORIGINAL ARTICLE
Morphological analysis of Spirulina sp. NCIM 5143 and Nostoc ellipsosporum NCIM 2786 and comparative characterization of associated enzymes through in silico approach Shreya Anand 1 & Koel Mukherjee 1 & Padmini Padmanabhan 1 Received: 21 January 2020 / Accepted: 20 May 2020 # Plant Science and Biodiversity Centre, Slovak Academy of Sciences 2020
Abstract Cyanobacteria are the only prokaryotes that can utilize energy sources from sunlight, electrons from water, carbon from the air, and have the capability of fixing nitrogen. The characterization of various cyanobacteria reveals the performance as well as growth differences when compared among different genus of cyanobacteria, which emphasizes the value of characterization in the cellular grounds. In this paper, we have performed a comparative study on the morphology, growth pattern, physiochemical properties as well as structural features of Spirulina sp. NCIM 5143 and Nostoc ellipsosporum NCIM 2786. The comparison is performed through microscopic images, FESEM and statistical study of cell growth by ANOVA. In silico approach was also carried out on the two enzymes present in both the cyanobacteria, Cyanophycin synthestase (EC: 6.3.2.29) and Nitrate reductase (EC: 1.7.1.1) by studying the physicochemical properties, sequential features and structural modeling. A descriptive profile study was completed on these microorganisms and their proteins that may help to interpret the molecular mechanism of the enzymatic reaction of 1, 3-Propanediol (PDO) production in the future. Keywords ANOVA . Cyanobacteria . FESEM . 1, 3-Propanediol (PDO . Structural modeling
Introduction Cyanobacteria form the developmentally diverse as well as morphologically stable group of prokaryotes (Stanier and Cohen-Bazire 1977). They consist of varied types of differentiated cells, including unicellular, colonial, as well as filamentous strains (Whitton and Potts 2007; Schirrmeister et al. 2013). It has led to a substantial dramatic modification in the living patterns of anaerobic to aerobic environments (Waterbury 2006). Cyanobacteria lack nuclei as well as other organelles; they possess a peptidoglycan cell wall, which is a characteristic
* Padmini Padmanabhan [email protected] Shreya Anand [email protected] Koel Mukherjee [email protected] 1
Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India
of a gram-negative bacteria (Whitton and Potts 2007). Cyanobacteria can be either unicellular, filamentous, or colonial; each of the filament has a mucilage sheath and some cellular strands known as trichomes. If the trichome is single filaments, then they are known as either homocystous or heterocystous (Olson 2006). The undifferentiated filaments are known as homocystous for example, Oscillatoria sp. and the differentiated filaments are known as heterocystous, i.e., which have heterocysts, for example, Nostoc sp. Some cyanobacteria like Spirulina sp. have a spirally coiled filament while some developed colonie
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