Morphological and molecular screening of rice germplasm lines for low soil P tolerance
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ORIGINAL ARTICLE
Morphological and molecular screening of rice germplasm lines for low soil P tolerance Ravindra Ramrao Kale1,2 • M. Anila2 • H. K. Mahadeva Swamy3 • V. P. Bhadana4 • Ch. V. Durga Rani1 • P. Senguttuvel2 • D. Subrahmanyam2 • S. K. Hajira2 • G. Rekha2 • M. Ayyappadass2 • B. Laxmiprasanna1 E. Punniakotti2 • M. B. V. N. Kousik2 • Swapnil Kulkarni2 • T. Dilip2 • Pragya Sinha2 • G. Harika2 • K. Pranathi2 • K. Chaitra2 • M. S. Anantha.2 • P. Brajendra2 • L. V. Subbarao2 • S. M. Balachandran2 • S. K. Mangrauhuia2 • Raman Meenakshi Sundaram2
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Received: 6 January 2020 / Accepted: 16 August 2020 Ó Society for Plant Biochemistry and Biotechnology 2020
Abstract Phosphorus (P) is an essential macronutrient to all crops including rice and it plays a key role in various plant activities and development. Low availability of P in the soils negatively, influences rice crop growth and causes significant yield loss. In the present study, we characterized a set of 56 germplasm lines for their tolerance to low soil P by screening them at low soil P and optimum soil P levels along with low soil P tolerant and sensitive check varieties. These lines were genotyped for the presence/absence of tolerant allele with respect to the major low soil P tolerance QTL, Pup1, using a set of locus specific PCR-based markers, viz., K46-1, K46-2, K52 and K46CG-1. High genetic variability was observed for various traits associated with low soil P tolerance. The yield parameters from normal and low soil P conditions were used to calculate stress tolerance indices and classify the genotypes according to their tolerance level. Out of the total germplasm lines screened, 15 lines were found to be tolerant to low soil P condition based on the yield reduction in comparison to the tolerant check, but most of them harbored the complete or partial Pup1 locus. Interestingly, two tolerant germplasm lines, IC216831 and IC216903 were observed to be completely devoid of Pup1 and hence they can be explored for new loci underlying low soil P tolerance. Keywords Low soil Phosphorus tolerance Pup1 IC216831 IC216903
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13562-020-00586-5) contains supplementary material, which is available to authorized users. & Raman Meenakshi Sundaram [email protected] 1
Institute of Biotechnology (IBT), PJTSAU, Rajendranagar, Hyderabad 500030, India
2
Crop Improvement Division, ICAR-Indian Institute of Rice Research (IIRR), Rajendranagar, Hyderabad 500030, India
3
ICAR-Sugarcane Breeding Institute (SBI), Coimbatore 641007, India
4
ICAR-Indian Institute of Agricultural Biotechnology (IIAB), Ranchi 834010, India
Rice is one of the most important cereal crops and occupies second prominent position in global agriculture. Imminent water scarcity, decrease in arable land, the constant battle against new emerging pathogens, pests and possible adverse effects of climate change will pose a great challenge for rice researchers in the near future (Khush and
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