MtCOPT2 is a Cu + transporter specifically expressed in Medicago truncatula mycorrhizal roots
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SHORT NOTE
MtCOPT2 is a Cu+ transporter specifically expressed in Medicago truncatula mycorrhizal roots Marta Senovilla 1,2 & Isidro Abreu 1,2,3 Manuel González-Guerrero 1,2
&
Viviana Escudero 1,2
&
Custodia Cano 4 & Alberto Bago 4 & Juan Imperial 5 &
Received: 8 January 2020 / Accepted: 7 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Arbuscular mycorrhizal fungi are critical participants in plant nutrition in natural ecosystems and in sustainable agriculture. A large proportion of the phosphorus, nitrogen, sulfur, and transition metal elements that the host plant requires are obtained from the soil by the fungal mycelium and released at the arbuscules in exchange for photosynthates. While many of the plant transporters responsible for obtaining macronutrients at the periarbuscular space have been characterized, the identities of those mediating transition metal uptake remain unknown. In this work, MtCOPT2 has been identified as the only member of the copper transporter family COPT in the model legume Medicago truncatula to be specifically expressed in mycorrhizal roots. Fusing a Cterminal GFP tag to MtCOPT2 expressed under its own promoter showed a distribution pattern that corresponds with arbuscule distribution in the roots. When expressed in tobacco leaves, MtCOPT2-GFP co-localizes with a plasma membrane marker. MtCOPT2 is intimately related to the rhizobial nodule-specific MtCOPT1, which is suggestive of a shared evolutionary lineage that links transition metal nutrition in the two main root endosymbioses in legumes. Keywords Copper transport . COPT . Medicago truncatula . Plant nutrition . Rhizophagus irregularis
Introduction Arbuscular mycorrhizal fungi (AMF) facilitate nutrient uptake by a majority of plants (Smith and Smith 2011). Through their branched absorbing structures (Bago et al. 1998), AMF take up phosphates, nitrogen compounds, sulfates, and transition metals from soils and deliver them along their coenocytic mycelium to the host plant (Casieri et al. 2013; di
Fossalunga and Novero 2019; Garcia et al. 2016; GuerreroGalan et al. 2018; Wipf et al. 2019). This impact of AMF on plant nutrition cannot be overstated: typically a large part of total plant phosphorus (Yang et al. 2012) and up to one-third of plant nitrogen (Govindarajulu et al. 2005) are provided through this mycorrhizal pathway, with reports indicating that AMF participate in the uptake of at least 50% of other essential elements (Allen and Shachar-Hill 2009; Watts-Williams
Marta Senovilla, Isidro Abreu and Viviana Escudero contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00572-020-00987-3) contains supplementary material, which is available to authorized users. * Isidro Abreu [email protected] * Manuel González-Guerrero [email protected] 1
Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón (Madrid), Spain
2
Escuela Técnica Superior de
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