Functional Genomics in Medicago truncatula Methods and Protocols

This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also descri

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Luis A. Cañas · José Pío Beltrán Editors

Functional Genomics in Medicago truncatula Methods and Protocols

Methods

in

M o l e c u l a r B i o lo g y

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Functional Genomics in Medicago truncatula Methods and Protocols

Edited by

Luis A. Cañas and José Pío Beltrán CSIC-UPV, Institute for Plant Cell and Molecular Biology (IBMCP), Valencia, Spain

Editors Luis A. Cañas CSIC-UPV Institute for Plant Cell and Molecular Biology (IBMCP) Valencia, Spain

José Pío Beltrán CSIC-UPV Institute for Plant Cell and Molecular Biology (IBMCP) Valencia, Spain

ISSN 1064-3745     ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8632-3    ISBN 978-1-4939-8633-0 (eBook) https://doi.org/10.1007/978-1-4939-8633-0 Library of Congress Control Number: 2018946782 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface Functional genomics in Medicago truncatula has been proved to be useful for genetic studies to resolve specific developmental or metabolic processes that are legume-specific or shown important differences with those described in Arabidopsis thaliana, the most studied model plant. Recent advances in the understanding of nitrogen fixation, nodulation, compound leaf, inflorescence and plant architecture, floral development, pod formation, secondary metabolites biosynthesis, and other developmental and metabolic aspects have been achieved based on the analysis of