Mycochemical composition and insecticidal bioactivity of Algerian desert truffles extract against two stored-product ins
- PDF / 1,090,446 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 75 Downloads / 202 Views
ORIGINAL ARTICLE
Mycochemical composition and insecticidal bioactivity of Algerian desert truffles extract against two stored‑product insects: Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) Samir Neggaz1 · Mohammed Chenni2 · Fatima El‑Houaria Zitouni‑Haouar1 · Xavier Fernandez3 Received: 1 July 2020 / Accepted: 7 October 2020 © King Abdulaziz City for Science and Technology 2020
Abstract The present study aimed to highlight the insecticidal activity of the Algerian desert truffle Terfezia claveryi Chatin against two post-harvest pests of rice and other cereal products, the rice weevil Sitophilus oryzae (L.), and the lesser grain borer Rhyzopertha dominica (F.), using direct contact application method with the filter paper impregnation technique. Soxhlet apparatus was used for extraction with chloroform as solvent. The major compounds in chloroform extract of T. claveryi were identified using gas chromatography–mass spectrometry (GC–MS). The two-way ANOVA was used for statistical analysis to determine the exposure time and doses with the greatest insecticidal activity. Findings of this study revealed that the major compounds were fatty acids: linoleic acid, methyl ester (14.84%), 11,14-eicosadienoic acid, methyl ester (11.55%), oleic acid, methyl ester (7.1%), and palmitic acid (6.96%). However, chamazulene (0.88%) was found to be minor compound. Our results describe for the first time the presence of chamazulene in desert truffle. The result showed also that the most potent insecticidal activity of chloroform extract of T. claveryi was found towards S. oryzea with L D50 value of 162.11 µg/ mL. For concentrations of 250 and 300 µg/mL, this extract was able to eradicate 96.65 ± 1.15% and 100.0 ± 0.0% of S. oryzea after 24 h of exposure. However, it caused only 47.24 ± 1.15% and 50.66 ± 1.15% of mortality of R. dominica, respectively, after 6 days. This work offers promising prospects for the use of desert truffles extracts as a potential insecticidal agent for improving quality and safety of stored foods against damage caused by stored-product pests. Keywords Bioinsecticides · Terfezia claveryi · Coleoptera · Soxhlet extraction · GC–MS analysis · Contact toxicity assay
Introduction Stored grains, cereals, and their products are important sources of food in the world, and thus, their effective conservation is essential for subsistence of mankind (Stejskal et al. 2015). Cereal farming is of strategic importance, since it is the basis of the country’s food security. Durum and soft wheat are the most widely grown cereals for human consumption. In Algeria, local cereals may be more or less sensitive to storage insect attacks which, depending on the year, cause losses of up to 20% in Africa (Fao 2012). The rice weevil Sitophilus oryzae (L.) and the lesser grain borer Rhyzopertha dominica (F.) cause significant economic * Samir Neggaz [email protected]; neggaz.samir@univ‑oran1.dz Extended author information available on the last page of the article
Data Loading...
