NAIP expression increases in a rat model of liver mass restoration
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ORIGINAL PAPER
NAIP expression increases in a rat model of liver mass restoration Julio Plaza‑Díaz1,2,3,4 · Ana I. Álvarez‑Mercado1,2,3 · Cándido Robles‑Sánchez1,2 · Miguel Navarro‑Oliveros3 · Virginia Morón‑Calvente5 · Sofía Toribio‑Castelló6 · María José Sáez‑Lara2,3,7 · Alex MacKenzie4,8 · Luis Fontana1,2,3 · Francisco Abadía‑Molina2,9 Received: 28 July 2020 / Accepted: 13 November 2020 © Springer Nature B.V. 2020
Abstract The neuronal apoptosis inhibitory protein (NAIP) is a constituent of the NLRC4 inflammasome, which plays a key role in innate immunity, and an antiapoptotic protein. Recently, we reported the previously undescribed role of NAIP in cell division. The liver is one of the body’s most actively regenerative organs. Given the novel mitotic role of NAIP, we examined its expression in hepatic mass restoration. The major liver lobe of Wistar rats was removed, and samples from both newly formed liver tissue, assessed by positive Ki67 immunostaining, and the remnant, intact liver lobes from hepatectomized rats were taken 3 and 7 days after surgery. Naip5 and Naip6 mRNA levels were significantly higher in regenerating hepatic tissue than in intact liver lobe tissue, and this increase was also observed at the protein level. Naip5 and Naip6 mRNA in situ hybridization showed that this increase occurred in the hepatic parenchyma. The histology of the regenerated liver tissue was normal, with the exception of a noticeable deficiency of hepatic lobule central veins. The results of this study suggest the involvement of NAIP in liver mass restoration following partial hepatectomy. Keywords NAIP · Liver mass restoration · Hepatectomy · Cell proliferation
Introduction The Inhibitor of Apoptosis Protein (IAP) family was discovered more than twenty years ago. In the intervening two decades, the roles of IAP family members in multiple cellular processes, such as apoptosis, cellular proliferation (Abadia-Molina et al. 2017; Mita et al. 2008), signal transduction, immunity and heavy metal homeostasis (Bertrand Luis Fontana and Francisco Abadía-Molina have contributed equally to this work. * Francisco Abadía‑Molina [email protected] 1
Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, 18071 Granada, Spain
2
Institute of Nutrition and Food Technology “José Mataix”, Biomedical Research Center, Avda. del Conocimiento S/N, Armilla, 18016 Granada, Spain
3
Instituto de Investigación Biosanitaria Ibs.GRANADA, Avda. de Madrid 15, 18012 Granada, Spain
4
Children’s Hospital of Eastern Ontario Research Institute, Ottawa, ON K1H 8L1, Canada
et al. 2009; Beug et al. 2012; Conte et al. 2006; Srinivasula and Ashwell 2008), have been delineated. Neuronal Apoptosis Inhibitory Protein (NAIP), the founding member of the mammalian IAP family (Liston et al. 1996; Roy et al. 1995), is composed of three zinc-binding baculovirus IAP repeat (BIR) domains, a nucleotide binding and oligomerization (NOD) domain, and a leucine-rich repeat domain (LRR). NAIP is also a mem
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