Nanofomulation of zinc oxide and chitosan zinc sustain oxidative stress and alter secondary metabolite profile in tobacc
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ORIGINAL ARTICLE
Nanofomulation of zinc oxide and chitosan zinc sustain oxidative stress and alter secondary metabolite profile in tobacco Kinjal V. Patel1 · Manoj Nath2 · Megha D. Bhatt3 · Anoop K. Dobriyal4 · Deepesh Bhatt1 Received: 21 May 2020 / Accepted: 5 October 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Advancement in nanotechnology has improved ways for large-scale production and characterization of nanoparticles of physiologically important metals. The current study explores the impact of Zinc Oxide Nanoparticles (ZnO-NP) and Chitosan-Zinc oxide nano-bioformulation (CH-ZnO) in tissue culture raised callus of Nicotiana benthamiana. Results indicated augmented biomass in CH-ZnO treated callus, while a reduced biomass was observed in ZnO-NP treated callus, at all the concentrations tested. Higher chlorophyll and carotenoid content were recorded in callus treated with 800 ppm CH-ZnO as compared to ZnO-NP treated callus. A higher accumulation of proline was observed in CH-ZnO treated callus when compared to ZnO-NP treatment, which was significantly higher at 50, 200 and 400 ppm CH-ZnO treatment. A maximum reduction in malondialdehyde (MDA) content was recorded at 800 ppm, for both the nano-formulations tested. Likewise, a significant reduction in the H2O2 levels was observed in all the treatments, while the callus treated with 400 ppm ZnO-NP and 800 ppm CH-ZnO recorded the highest reduction. Phenylalanine Ammonia-Lyase (PAL), activity increased significantly in callus treated with 400 ppm concentration for both ZnO-NP and CH-ZnO with respect to control. An increased level of tannin and nicotine were recorded in callus supplemented with 50, 200 and 400 ppm CH-ZnO. Notably, a significant decline of 94 and 52% in tannin content and 25 and 50% in nicotine content was recorded in the callus treated with 800 ppm CH-ZnO and ZnO-NP, respectively. The findings of this study suggest that an optimized dosage of these nano-bioformulations could be utilized to regulate the nicotine content and stress tolerance level. Keywords Chitosan · Nano biotechnology · Nicotine · Oxidative stress · Zinc oxide nano-bioformulation
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02469-x) contains supplementary material, which is available to authorized users. * Deepesh Bhatt [email protected] 1
Department of Biotechnology, Shree Ramkrishna Institute of Computer Education and Applied Sciences, Affiliated to Veer Narmad South Gujarat University, Surat, Gujarat, India
2
ICAR-Directorate of Mushroom Research, Chambaghat, Solan, Himachal Pradesh, India
3
G B Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
4
HNB Garhwal Central University, Pauri campus, Garhwal, Uttarakhand, India
Nanotechnology is a promising technique holding a dominant position in transforming agriculture and food production in a sustainable manner. Nanoparticles have potential applications in agriculture system viz., con
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