Nitric Oxide Methods and Protocols
The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in
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MO L E C U L A R BI O L O G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
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Nitric Oxide Methods and Protocols
Edited by
Helen O. McCarthy School of Pharmacy, Queen’s University Belfast, Northern Ireland, UK
Jonathan A. Coulter School of Pharmacy, Queen’s University Belfast, Northern Ireland, UK
Editors Helen O. McCarthy School of Pharmacy Queen’s University, Belfast Northern Ireland BT9 7BL, UK [email protected]
Jonathan A. Coulter School of Pharmacy Queen’s University, Belfast Northern Ireland BT9 7BL, UK [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61737-963-5 e-ISBN 978-1-61737-964-2 DOI 10.1007/978-1-61737-964-2 Springer New York Dordrecht Heidelberg London © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface This book forms part of the highly acclaimed Methods in Molecular BiologyTM series, which aims to provide a detailed reference manual giving a step-by-step approach to reproduce various complex protocols within your own laboratory. For each volume in this series, editors have included the most interesting and relevant methodologies published in the field in recent years, thereby providing access to the most novel experimental approaches. In addition, this series also provides a detailed notes section which documents specific information relating to particularly challenging aspects of a methodology. The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. This volume considers two of the main aspects of nitric oxide research. Section I of the book includes a review from an expert in tumor radiosensitization induced by various novel compounds, including nitric oxide. The review covers multiple facets of nitric oxide including its role in addiction, the cardiovascular system, the nervous system, and cancer.
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