Cytoskeleton Methods and Protocols Methods and Protocols
The third edition of this volume focuses on experimental models that are useful for investigating various aspects of cytoskeleton structure and function. Animal, plant, protist, and fungal models highlight twenty-four chapters that provide detailed protoc
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Ray H. Gavin Editor
Cytoskeleton Methods and Protocols Third Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Cytoskeleton Methods and Protocols Third Edition
Edited by
Ray H. Gavin Brooklyn College, Brooklyn, NY, USA
Editor Ray H. Gavin Brooklyn College Brooklyn, NY, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3123-1 ISBN 978-1-4939-3124-8 (eBook) DOI 10.1007/978-1-4939-3124-8 Library of Congress Control Number: 2015947776 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface It has been 15 years since the publication of the first edition of Cytoskeleton Methods and Protocols, and in reflection, I am mindful of the many advances in our understanding of the cytoskeleton over the last four decades. The earliest images of the cytoskeleton revealed a complex network of seemingly interconnected arrays of various fibers, some of which appeared filamentous, and others appeared tubular. Synergy of cytoskeleton fibers was not widely accepted, and the notion was slow to gain acceptance. However, convincing evidence for synergistic action among cytoskeleton fibers as well as more detailed knowledge about their structure and function continually emerges. The dramatic interplay of cytoskeleton components is aptly illustrated in the image of a HeLa cell (Fig. 1). Continuing advances in cell imaging reflect refinements in various forms of microscopy and have significantly expanded our understanding of cytoskeleton structure and function. Technological advances have led to development of magne
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