Nitric oxide, prostaglandins and angiotensin II in the regulation of renal medullary blood flow during volume expansion
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ORIGINAL PAPER
Nitric oxide, prostaglandins and angiotensin II in the regulation of renal medullary blood flow during volume expansion Carol Moreno & María T. Llinás & Francisca Rodriguez & Juan M. Moreno & F. Javier Salazar
Received: 2 June 2015 / Accepted: 17 November 2015 # University of Navarra 2015
Abstract Regulation of medullary blood flow (MBF) is essential in maintaining renal function and blood pressure. However, it is unknown whether outer MBF (OMBF) and papillary blood flow (PBF) are regulated independently when extracellular volume (ECV) is enhanced. The aim of this study was to determine whether OMBF and PBF are differently regulated and whether there is an interaction between nitric oxide (NO), prostaglandins (PGs) and angiotensin II (Ang II) in regulating OMBF and PBF when ECV is enhanced. To achieve these goals, OMBF and PBF were measured by laserDoppler in volume-expanded rats treated with a cyclooxygenase inhibitor (meclofenamate, 3 mg/kg) and/or a NO synthesis inhibitor (L-nitro-arginine methyl ester (L-NAME), 3 μg/kg/min) and/or Ang II (10 ng/kg/ min). OMBF was unchanged by NO or PGs synthesis inhibition but decreased by 36 % (P < 0.05) when
M. T. Llinás : F. Rodriguez : J. M. Moreno : F. J. Salazar (*) Department of Physiology, School of Medicine, University of Murcia, Murcia 30100, Spain e-mail: [email protected] M. T. Llinás : F. Rodriguez : J. M. Moreno : F. J. Salazar Regional Campus of International Excellence BMare Nostrum^, Murcia, Spain M. T. Llinás : F. Rodriguez : J. M. Moreno : F. J. Salazar Instituto Murciano de Investigación Biomédica, Murcia, Spain C. Moreno Cardiovascular and Metabolic Diseases, MedImmune, Cambridge, UK
L-NAME and meclofenamate were infused simultaneously. PBF was similarly reduced by L-NAME (12 %), meclofenamate (17 %) or L-NAME+meclofenamate (19 %). Ang II did not modify OMBF, but it led to a similar decrease (P
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