Novel xylanase producing Bacillus strain X2: molecular phylogenetic analysis and its application for production of xyloo
- PDF / 2,548,247 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 69 Downloads / 166 Views
ORIGINAL ARTICLE
Novel xylanase producing Bacillus strain X2: molecular phylogenetic analysis and its application for production of xylooligosaccharides Chandrabhan Dhruw1 · Khadim Husain1 · Vyas Kumar1 · Vijay Chintaman Sonawane1 Received: 25 October 2019 / Accepted: 24 June 2020 © King Abdulaziz City for Science and Technology 2020
Abstract A Bacillus strain X2 that produced extracellular endo-xylanase (GH 11) (EC: 3.2.1.8) was isolated from the soil of the Northeast India region. This aerobic culture was Gram positive and endospore forming. Chemotaxonomic characterization showed variance with the fatty acid profile of related species in the Bacillus subtilis group. In Bacillus strain X2, distinct occurrence of iso-C14:0 lipids is absent in other related species. The 16S rRNA gene sequence homology showed 99% similarity with Bacillus subtilis subsp. inaquosorum. The phylogenetic analysis by the multilocus sequence analysis (MLSA) of the nucleotide sequence of six concatenated genes (16S rRNA, groEL, gyrA, polC, purH and rpoB) resolved the taxonomic position of the Bacillus strain X2 in the Bacillus subtilis subsp. group. The MLSA showed that it is a member of a clade that includes Bacillus subtilis subsp. stercoris. In in silico DNA–DNA hybridization (DDH), the highest matching score was obtained with Bacillus subtilis subsp. stercoris (87%). The in silico DDH of the genome (G + C 43.7 mol %) shared 48.5%, with Bacillus subtilis subsp. inaquosorum. The MLSA phylogenetic tree and the highest degree of DNA hybridization, indicating that it belongs to the Bacillus subtilis subspecies stercoris. Keywords Xylanolytic bacteria · Polyphasic characterization · Bacillus subtilis · Northeast india · Xylanase · Xylooligosaccharides Abbreviations MALDI-ToF Matrix-assisted laser desorption ionizationtime of flight XOS Xylooligosaccharides MLSA Multilocus sequence analysis FAMEs Fatty acid methyl esters
Introduction The Bacillus genus comprises a rod-shaped aerobic, endospore-forming group of bacteria that have been classified under the phylum Firmicutes (Logan and De Vos 2009). They are ubiquitous in nature and are found in Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02322-1) contains supplementary material, which is available to authorized users. * Vijay Chintaman Sonawane [email protected] 1
BERPDC, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India
diverse ecological habitats due to their ability to metabolize a wide range substrates and to form endospores in adverse conditions (Zwick et al. 2012). Recently, many new Bacillus species which were classified as Bacillus subtilis have been reclassified as B. atrophaeus, B. velezensis, B. tequilensis and more (Nakamura 1989; Roberts et al. 1994, 1996; Palmisano et al. 2001; Gatson et al. 2006; Wang et al. 2007, 2008). A xylanolytic Bacillus strain X2 was isolated from decaying wood samples collected from the northeast state of India, Assam. This aerobic culture was Gram po
Data Loading...
