Cellulase and Xylanase Production by the Mexican Strain Talaromyces stollii LV186 and Its Application in the Saccharific
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Cellulase and Xylanase Production by the Mexican Strain Talaromyces stollii LV186 and Its Application in the Saccharification of Pretreated Corn and Sorghum Stover Montserrat Orencio-Trejo 1,2 & Jessica Torres-Granados 1 & Arelis Rangel-Lara 1 & Esmeralda Beltrán-Guerrero 1 & Santos García-Aguilar 1 & Cessna Moss-Acosta 3 & Humberto Valenzuela-Soto 1,4 & Susana De la Torre-Zavala 1,2 & Argel Gastelum-Arellanez 2,5 & Alfredo Martinez 3 & Axel Tiessen 1 & Edith Diaz-Mireles 1 & Edmundo Lozoya-Gloria 1
# Springer Science+Business Media New York 2016
Abstract A Mexican strain of Talaromyces stollii LV186 was isolated from decaying pretreated corn stover. The production of cellulase and xylanase enzyme cocktails was evaluated with corn and sorghum stover used as inducers in a mineral medium. The volumetric and specific activities of T. stollii LV186 were compared with the values produced by Trichoderma reesei ATCC 26921 in a time-course experiment. After the submerged culture and a posterior ultrafiltration stage, the enzyme complexes were evaluated over acid-pretreated corn or sorghum stover in baffled flasks under controlled temperature and agitation conditions, and hydrolysis levels of 30 and 39 % of the theoretical maximum were obtained after only 72h reactions, for each substrate. A side-by-side comparison showed a better ratio of endoglucanase to cellobiohydrolase
to β-glucosidase and of xylanase to β-xylosidase enzymes in T. stollii than in T. reesei ATCC 26921. Furthermore, the hydrolysis of pretreated corn and sorghum stover achieved by T. stollii is significantly higher compared with that of a commercial cocktail from T. reesei ATCC 26921 (Celluclast). Therefore, the T. stollii LV186 strain is a good candidate for the hydrolysis of complex lignocellulose substrates. To the authors’ knowledge, this study is the first to describe the cellulolytic and hemicellulolytic activities produced by a T. stollii strain.
* Montserrat Orencio-Trejo [email protected]
Introduction
1
Departamento de Ingeniería Genética, CINVESTAV IPN Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, C.P. 36821 Irapuato, Gto, Mexico
2
Present address: Instituto de Biotecnología de la Universidad Autónoma de Nuevo León, Av. Pedro de Alba y Manuel L. Barragán, Ciudad Universitaria, C.P. 66450 San Nicolás de los Garza, N.L, Mexico
3
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, 62250 Cuernavaca, Morelos, Mexico
4
Present address: Departamento de Plásticos en la Agricultura, Centro de Investigación en Química Aplicada, Boulevard Enrique Reyna Hermosillo 140, C.P. 25294 Saltillo, Coahuila, Mexico
5
Departamento de Biotecnología y Bioquímica, CINVESTAV IPN Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, C.P. 36821 Irapuato, Gto, Mexico
Keywords Cellulases . Xylanases . Enzymatic hydrolysis . Corn and sorghum stover . Talaromyces stollii
Among potential alternative energy sources, lignocellulose
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