Nucleic Acid Transfection

Gene Delivery into Mammalian Cells: An Overview on Existing Approaches Employed In Vitro and In Vivo, by Peter Hahn and Elizabeth Scanlan *Strategies for the Preparation of Synthetic Transfection Vectors, by Asier Unciti-Broceta, Matthew N. Bacon, and Mar

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Topics in Current Chemistry

Editorial Board: A. de Meijere K.N. Houk C.A. Hunter H. Kessler J.-M. Lehn S.V. Ley M. Olivucci J. Thiem B.M. Trost M. Venturi P. Vogel H. Wong H. Yamamoto l

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Topics in Current Chemistry Recently Published and Forthcoming Volumes

Nucleic Acid Transfection Volume Editors: Bielke, W., Erbacher, C. Vol. 296, 2010 Carbohydrates in Sustainable Development II Volume Editors: Rauter, A.P., Vogel, P., Queneau, Y. Vol. 295, 2010 Carbohydrates in Sustainable Development I Volume Editors: Rauter, A.P., Vogel, P., Queneau, Y. Vol. 294, 2010 Functional Metal-Organic Frameworks: Gas Storage, Separation and Catalysis Volume Editor: Schro¨der, M. Vol. 293, 2010 C-H Activation Volume Editors: Yu, J.-Q., Shi, Z. Vol. 292, 2010 Asymmetric Organocatalysis Volume Editor: List, B. Vol. 291, 2010 Ionic Liquids Volume Editor: Kirchner, B. Vol. 290, 2010 Orbitals in Chemistry Volume Editor: Inagaki, S. Vol. 289, 2009 Glycoscience and Microbial Adhesion Volume Editors: Lindhorst, T.K., Oscarson, S. Vol. 288, 2009 Templates in Chemistry III Volume Editors: Broekmann, P., Do¨tz, K.-H., Schalley, C.A. Vol. 287, 2009

Tubulin-Binding Agents: Synthetic, Structural and Mechanistic Insights Volume Editor: Carlomagno, T. Vol. 286, 2009 STM and AFM Studies on (Bio)molecular Systems: Unravelling the Nanoworld Volume Editor: Samorı`, P. Vol. 285, 2008 Amplification of Chirality Volume Editor: Soai, K. Vol. 284, 2008 Anthracycline Chemistry and Biology II Mode of Action, Clinical Aspects and New Drugs Volume Editor: Krohn, K. Vol. 283, 2008 Anthracycline Chemistry and Biology I Biological Occurence and Biosynthesis, Synthesis and Chemistry Volume Editor: Krohn, K. Vol. 282, 2008 Photochemistry and Photophysics of Coordination Compounds II Volume Editors: Balzani, V., Campagna, S. Vol. 281, 2007 Photochemistry and Photophysics of Coordination Compounds I Volume Editors: Balzani, V., Campagna, S. Vol. 280, 2007 Metal Catalyzed Reductive C–C Bond Formation A Departure from Preformed Organometallic Reagents Volume Editor: Krische, M.J. Vol. 279, 2007

Nucleic Acid Transfection

Volume Editors: Wolfgang Bielke  Christoph Erbacher

With Contributions by A. Ahmad  M.N. Bacon  K. Berg  M. Berstad  N.F. Bouxsein  C. Bra¨uchle  M. Bradley  M. Caldero´n  H.M. Evans  K.K. Ewert  W. Fischer  R. Haag  P. Hahn  I. Hedfors  A. Høgset  R. Koynova  C.S. McAllister  P.M. McLendon  L. Prasmickaite  T.M. Reineke  N. Ruthardt  C.R. Safinya  E.E. Salcher  C.E. Samuel  E. Scanlan  P.K. Selbo  A. Sizovs  S. Srinivasachari  B. Tenchov  A. Unciti-Broceta  E. Wagner  A. Weyergang  A. Zidovska

Editors Dr. Wolfgang Bielke QIAGEN GmbH Qiagen Str. 1 40724 Hilden Germany [email protected]

Dr. Christoph Erbacher QIAGEN GmbH Division Corporate Research Qiagen Str. 1 40724 Hilden Germany [email protected]

ISSN 0340-1022 e-ISSN 1436-5049 ISBN 978-3-642-16429-3 e-ISBN 978-3-642-16430-9 DOI 10.1007/978-3-642-16430-9 Springer Heidelberg Dordrecht London New York Library of Con