Oleanolicacid-Chitosan Nanocomplex Induced Apoptotic Cell Death Through Mitochondrial Dysfunction in Human Lung Carcinom
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ORIGINAL PAPER
Oleanolicacid-Chitosan Nanocomplex Induced Apoptotic Cell Death Through Mitochondrial Dysfunction in Human Lung Carcinoma: An Improved Synergetic Drug System for Cancer Therapy Abulimiti Abulaiti1 • Wei Sun1 • Adili Salai1 • Xiaohong Sun1 • Waresijiang Yibulayin1 • Yunfei Gao1 Received: 4 June 2020 / Accepted: 3 November 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Lung carcinoma is a forceful type of malignancy, having great elevated pace of morbidity and mortality with limited therapeutic options. The goal of current examination was to reveal the anticancer potential embedded in the oleanolic acid conjugated chitosan nanocomplex (OAC) in Human lung cancer cells (A-549). The OAC complex was characterized with DLS, Zeta potential, FTIR and SEM analysis. We found that the cytotoxic efficacy of OAC in Human lung cancer cells (A549) was dose dependent and also shows insignificant cytotoxicity towards to Human normal lung epithelial normal cells IMR-90. The influence of OAC nanocomplex on cell cycle phase distribution and also the mitochondrial membrane potential was assessed using flowcytometry. The results revealed that oleanolic acid nanocomplex system induced apoptotic cell death in a dose dependent manner. Subsequently the acridine orange and ethidium bromide staining (AO/ EtBr) treatment was done with different concentrations of OAC nanocomplex had initiated a characteristic morphological alterations associated with apoptosis. The present findings concluded that OAC nanocomplex treatment brought about the loss in integrity of mitochondrial membrane potential, which occurred in treated human lung cancer cells. Thus the oleanolic acid nanocomplex could be utilized as a therapeutic mediator in the treatment of cancers in humans. Keywords Lung cancer Apoptosis Cell cycle Oleanolicacid nanocomplex Flowcytometry
Introduction Cancer is a top devastating disease, known to be the one affecting worldwide irrespective of the gender and age and cause increased death. Each year with the increase in about 10 million new cancer cases, there will be an expansion in the cancer related deaths to about * 13.1 million continuously 2030 as assessed by the World Health Organization (WHO) [1]. Among the malignant cancers, the lung cancer or carcinomais considered as the second leading disease found profusely in both men (followed by prostate cancer) and women (followed by breast cancer)and are known to be the pivotal reason for death of many patients [2]. Lung cancer is a commonest and group disease with the
& Adili Salai [email protected] 1
Department of Thoracic Surgery, Xinjiang Tumor Hospital, Urumqi 830011, China
increased mortality rate in both men and women. Based on the therapeutic determination, the lung cancer is classified into two sub-types which include non-small cell lung carcinoma (NSCLC) and small-cell lung carcinoma (SCLC). The former one is considered to be the foremost cancer affecting many people and
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