One-Step Core/Multishell Quantum Dots-Based Fluoroimmunoassay for Screening of Deoxynivalenol in Maize
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One-Step Core/Multishell Quantum Dots-Based Fluoroimmunoassay for Screening of Deoxynivalenol in Maize Xiya Zhang 1,2 & Xuezhi Yu 2 & Jianyi Wang 2 & Qi Wang 3 & Hui Meng 4 & Zhanhui Wang 2 Received: 18 December 2017 / Accepted: 5 February 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018
Abstract Deoxynivalenol (DON) is a protein synthesis inhibitor produced by the Fusarium species, which frequently contaminates grains used for human or animal consumption, thereby necessitating their effective monitoring and control. Antibody-based immunoassays provide efficient and cheap methods for screening this mycotoxin. To achieve high sensitivity and avoid enzymatic reaction, a new highly sensitive monoclonal antibody (MAb) and a fluorescent probe consisting of amino-functionalized core/multishell quantum dots (QDs), CdSe/CdS/Cd0.5Zn0.5S/ZnS, bio conjugated to 3-HS-DON-BSA were prepared; then, a sensitive and reliable direct competitive fluorescent-labeled immunosorbent assay (dcFLISA) was developed. Under optimized conditions, the 50% inhibition concentration (IC50) value of the dcFLISA was 5.6 μg/kg with limit of detection of 12.2 μg/kg in maize samples. The recoveries ranged from 77.3 to 84.3% with coefficients of variation (CV%) values below 10%. Good correlation between the dcFLISA and HPLC-MS/MS results in the naturally contaminated maize demonstrated that the dcFLISA was a rapid and sensitive with high-performance and reliable method for the detection of DON. The potential of this method to function as a simple and efficient strategy for small molecule on-site screening might be applied in the future. Keywords Monoclonal antibody . Deoxynivalenol . Quantum dots . Direct competitive fluorescent-labeled immunosorbent assay
Introduction The food contamination by mycotoxins is a serious problem all over the world and represents an important risk factor for human and animal health, as up to 70% of the world’s crop production may be contaminated (Streit et al. 2013). Deoxynivalenol (DON) is a common mycotoxin produced by some Fusarium species such as Fusarium graminearum * Zhanhui Wang [email protected] 1
Henan Province Engineering Research Center for Food Safety Control of Processing and Circulation, College of Food Science and Technology, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan 450002, China
2
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
3
Beijing Center for Physical and Chemical Analysis, Hatchery Building B, No. 7 middle Feng Xian Road, Haidian District, Beijing 100094, China
4
School of the Environment and Safety Engineering, Jiangsu university, 301 Xuefu Road, Zhenjiang, Jiangsu Province 212013, China
and Fusarium culmorum (Li et al. 2015). Corn is an important raw material for animals, which often contaminated with DON in European Union (EU), Asian-Pacific region, and USA (Binder et al. 2007). One study showed that about 30% of 50 random sampl
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