Oocyte Vitrification Temporarily Turns on Oxidation-Reduction Process Genes in Mouse Preimplantation Embryos
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REPRODUCTIVE BIOLOGY: ORIGINAL ARTICLE
Oocyte Vitrification Temporarily Turns on Oxidation-Reduction Process Genes in Mouse Preimplantation Embryos Gu Ruihuan 1 & Li Zhichao 1 & Guo Song 1 & Fu Jing 1 & Sun Yijuan 1
&
Sun Xiaoxi 1,2
Received: 30 June 2020 / Accepted: 28 September 2020 # Society for Reproductive Investigation 2020
Abstract We aim to understand how oocyte vitrification impacts subsequent mouse preimplantation embryo development at molecular level. We profiled transcriptomics of fertilized preimplantation embryos derived from mouse vitrified-warmed oocytes. Concomitantly, we evaluated epigenetic markers in fertilized preimplantation embryos. We found that oocyte vitrification did not affect the fertilization and cleavage process but delayed embryo development until blastocyst stage. RNA sequencing revealed that 1575 genes were profoundly altered in the 2-cell stage embryos developed from vitrified oocytes. The most significantly altered biological pathway was “oxidation-reduction process.” Such profound transcriptomics change was associated with decreased level of oocyte-specific histone H1FOO in zygote and 2-cell stage. Transcriptome alteration due to oocyte vitrification was less pronounced as embryos develop into the morula stage. Oocyte vitrification temporarily changes transcriptomics in early preimplantation embryos. Targeting oxidation-reduction pathway might be a potential therapeutic strategy to improve embryo quality and long-term embryo survival. Keywords Oocyte vitrification . Preimplantation embryo development . Single-cell RNA-seq . Epigenetic modification
Background Oocyte cryopreservation is a process used to preserve fertility in women who are about to lose their ovarian function and women who would like to freeze “young oocyte” for future use [1]. Growing needs for oocyte cryopreservation and in vitro fertilization (IVF) require appropriate oocyte vitrification in order to preserve optimal oocyte viability and functions. Due to the huge size, fragility, and intricate meiotic spindle structure of oocytes, oocyte vitrification is one of the
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s43032-020-00337-w) contains supplementary material, which is available to authorized users. * Sun Yijuan [email protected] * Sun Xiaoxi [email protected] 1
Shanghai Ji Ai Genetics & IVF Institute, Obstetrics & Gynecology Hospital, Fudan University, Shanghai, China
2
Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics & Gynecology Hospital, Fudan University, Shanghai, China
most challenging steps in the whole process [2]. With the advancement of vitrification technique, the survival rate and final live birth rate of IVF has been significantly improved in recent years. The major problem of applying this technique is whether it is safe. Given that the impact of oocyte vitrification is not completely understood [3], more data on the safety aspect of oocyte vitrification is needed before this technology could be routi
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