Optimisation of double-enzymatic extraction of arabinoxylan from fresh corn fibre
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ORIGINAL ARTICLE
Optimisation of double-enzymatic extraction of arabinoxylan from fresh corn fibre Fumin Ma1 • Xiaolei Li1 • Jiayu Yin2 • Lin Ma1 • Dan Li1
Revised: 18 April 2020 / Accepted: 29 April 2020 Ó Association of Food Scientists & Technologists (India) 2020
Abstract Enzymatic extraction of arabinoxylans (AXs) is an attractive and environmentally friendly extraction option, in which technical considerations (yield and purity) have been coupled with environmental concerns. Amano HC 90 and Cellulase were combined to evaluate their interactive effects on AX extraction from destarched, deproteinised bran (DSDPB). A response surface methodology was used to obtain the optimal extraction conditions. The experimental data fit well with the predicted values and the model adequately represented the actual relationship among the measured parameters. The extraction yield and AX content in the extract under optimal conditions (double-enzyme dose of 920 U/g, pH of 3.0, extraction temperature of 35.0 °C; extraction time of 6 h; and DSDPB to liquid ratio of 1:30) were 40.73 ± 0.09% and 75.88 ± 0.11%, respectively. The double-enzymatic extraction method of AX from fresh corn fibre was more efficient than the chemical method. Keywords Arabinoxylan Central composite rotatable design Enzymatic Fresh corn fibre
& Dan Li [email protected] 1
Key Laboratory of Agroproducts Processing Technology at Jilin Provincial Universities, Education Department of Jilin Provincial Government, College of Food Science and Engineering, Changchun University, Changchun 130022, Jilin, People’s Republic of China
2
School of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, People’s Republic of China
Introduction Lignocellulosic materials currently represent the largest biomass resource in the world. If separated into cellulose, xylan and lignin, these materials could be used for the production of food and bioenergy, which may be important given the limitations in available agricultural area and the future scarcity of fossil fuels (de Figueiredo et al. 2017). Corn fibre is one of the most abundantly produced lowvalue lignocellulosic materials in the world, and is composed mainly of testa, pericarp, the aleurone layer and residual sclerenchyma of the endosperm (Yadav et al. 2008; Rose et al. 2010; Ayala-Soto et al. 2014; Sa´rossy et al. 2013). Thus, comprehensive utilisation of corn bran is very important. The major components of corn fibre are hemicellulose, cellulose and lignin. Arabinoxylans (AXs) are mainly localised in endosperm cell walls, the aleurone layer and the pericarp of cereal grains. Corn fibre contains higher quantities of AXs than other cereal grains (Fadel et al. 2017). AXs are comprised of a linear ß-(1,4)-D-xylopyranose backbone and L-arabinofuranose residues as side chains on O-2 and/or O-3. Some of the arabinose moieties are ester-linked to the O-5 positions of hydroxycinnamic acids, such as ferulic acid, p-coumaric acid, and dimers and trimers of ferulic acid (Ayala-Soto et al. 2014
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