Organoids Stem Cells, Structure, and Function
This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids i
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Kursad Turksen Editor
Organoids Stem Cells, Structure, and Function
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Organoids Stem Cells, Structure, and Function
Edited by
Kursad Turksen Ottawa Hospital Research Institute, Ottawa, ON, Canada
Editor Kursad Turksen Ottawa Hospital Research Institute Ottawa, ON, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7616-4 ISBN 978-1-4939-7617-1 (eBook) https://doi.org/10.1007/978-1-4939-7617-1 Library of Congress Control Number: 2018965202 © Springer Science+Business Media, LLC, part of Springer Nature 2019 Open Access Chapter “Drug Sensitivity Assays of Human Cancer Organoid Cultures” is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). For further details see licence information in the chapter. This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface One of the outstanding challenges in regenerative medicine is how to instruct stem/early progenitor cells to progress through the appropriate steps to generate functional 3-dimensional organs. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. I have attempted to collect representative protocols that show the exciting advances that have been made over the last several years in this area. I am very grateful to the contributors who have shared their hard-won successes in this volume. Once again
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