OsHIPP24 is a Copper Metallochaperone Which Affects Rice Growth
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RESEARCH ARTICLE
OsHIPP24 is a Copper Metallochaperone Which Affects Rice Growth Guoqiang Chen1,2,3 · Shuo Xiong1,2 Received: 8 September 2020 / Revised: 23 October 2020 / Accepted: 27 October 2020 © Korean Society of Plant Biologist 2020
Abstract Copper (Cu) is a trace mineral nutrient essential for plant growth; consequently, its uptake, trafficking, and redistribution are precisely controlled. Currently, little is known about the underlying regulatory mechanisms in rice. In this work, we identified OsHIPP24 as a Cu metallochaperone in rice. Heterologous expression of OsHIPP24 in wild-type yeast (Saccharomyces cerevisiae) strain BY4741 increased accumulation of Cu, as well as cadmium (Cd). OsHIPP24 was expressed constitutively in most tissues of rice, including root, stem, leaf, leaf sheath, internode, rachis, and spikelet, and particularly high in root stele along with the vascular bundle of the shoot. Overexpression or knockout of OsHIPP24 in rice led to stunted growth and reduced plant height, regardless of Cu levels in the growth medium. Taken together, these observations indicated that the physiological roles of OsHIPP24 involve the delivery of metal micronutrients via vascular tissues, and that OsHIPP24 plays a crucial role in the growth of rice. Keywords OsHIPP24 · Copper · Cadmium · Metallochaperone · Rice Abbreviations Cu Copper Cd Cadmium COPT COPPER Transporter ZIP Zinc-regulated transporter, Iron-regulated transporter-like Protein family YSL Yellow Stripe-Like family NA Nicotianamine HMAs Heavy metal-transporting ATPases Cu/Zn SOD Cu/Zn superoxide dismutase HPPs HMA plant proteins HIPPs HMA isoprenylated plant proteins OE Overexpression ORF Open reading frame Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12374-020-09287-x) contains supplementary material, which is available to authorized users. * Guoqiang Chen [email protected] 1
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
3
Present Address: Jiangsu Ocean University, Lianyungang 222005, China
SD-Ura SD medium without uracil SG-Ura SG medium without uracil TF Trigger factor protein IPTG Isopropyl-beta-d-thiogalactopyranoside cv Cultivar qRT-PCR Quantitative real-time PCR
Introduction Copper (Cu) is an important microelement for all living things, as well as a structural component of many enzymes and active proteins. Cu participates in many kinds of a physiological process, including photosynthesis, electron transfer, antioxidant stress, and hormone signal transduction (Burkhead et al. 2009). In plants, Cu enters the cytoplasm mostly via COPT (COPPER Transporter) proteins, which participate in the Cu acquisition from the soil, Cu distribution in aboveground tissues, intercellular Cu storage, and pollen development (Klaumann et al. 2011; Jung et al. 2012; Garcia-Molina et al. 2013; Andrés-Colás et al. 2018). The Zinc-regulate
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