OsRbohB-mediated ROS production plays a crucial role in drought stress tolerance of rice
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ORIGINAL ARTICLE
OsRbohB‑mediated ROS production plays a crucial role in drought stress tolerance of rice Yi Shi1 · Yan‑Li Chang1 · Hai‑Tao Wu2 · Abdullah Shalmani1 · Wen‑Ting Liu1 · Wen‑Qiang Li1 · Jian‑Wei Xu2 · Kun‑Ming Chen1 Received: 8 June 2020 / Revised: 17 August 2020 / Accepted: 13 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message We found that a rice NADPH oxidase gene OsRbohB contributes drought tolerance and its functions are involved in the interaction of the OsRbohB-mediated ROS production and ABA signaling. Abstract The plasma membrane NADPH oxidases, also known as respiratory burst oxidase homologs, are the key producers of ROS under both normal and stress conditions in plants. However, their functions in rice development and stress tolerance are still under investigation. Here, we found that a rice NADPH oxidase gene OsRbohB, also named OsNOX1, is expressed in all tissues examined throughout the development stages with higher transcripts in leaves. The transcriptional expression of OsRbohB is also strongly stimulated by dehydration, salt and several phytohormonal treatments. Compared with widetype and the OsRbohB-overexpressing transgenic plants, osrbohB, a Tos17 insertion knockout mutant of OsRbohB, shows lower ROS production, abscisic acid (ABA) content and transcripts of a series of stress-related genes. The osrbohB mutant also exhibits lower seed germination rate, organ size and thousand seed weight, but higher stomatal aperture and sensitivity to drought. Moreover, a number of genes involved in plant development, stress response, transcriptional regulation, and particularly ABA signaling are differentially expressed in osrbohB plants under both normal growth and drought conditions. All these results suggest the roles of OsRbohB in drought tolerance of rice, which probably performed through the interaction of the OsRbohB-mediated ROS production and ABA signaling. Keywords NADPH oxidase · OsRbohB · Drought · ROS · ABA · Rice (Oryza sativa)
Introduction Communicated by Neal Stewart. Yi Shi and Yan-Li Chang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02603-2) contains supplementary material, which is available to authorized users.
Reactive oxygen species (ROS) burst is a common feature in plant response to a number of biotic and abiotic environmental stresses and even for normal growth regulation. Under normal growth conditions, the intracellular homeostasis of
* Kun‑Ming Chen [email protected]; [email protected]
Wen‑Qiang Li [email protected]
Yi Shi [email protected]
Jian‑Wei Xu [email protected]
Yan‑Li Chang [email protected]
1
State Key Laboratory of Crop Stress Biology in Arid Area/ College of Life Sciences, Northwest A&F University, Yangling 712100, Shanxi, China
2
College of Science, Northwest A&F University, Yangling 712100, Shanxi, China
Hai‑Tao Wu [email protected] Abdullah Shalmani abdullahshalamni@
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