Autophagy-Related Protein MAP1LC3C Plays a Crucial Role in Odontogenic Differentiation of Human Dental Pulp Cells
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ORIGINAL ARTICLE
Autophagy-Related Protein MAP1LC3C Plays a Crucial Role in Odontogenic Differentiation of Human Dental Pulp Cells Heui Seung Cho1 • Sam Young Park1 • Sun Mi Kim2 • Won Jae Kim1 Ji Yeon Jung1
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Received: 19 August 2020 / Revised: 27 September 2020 / Accepted: 13 October 2020 Ó The Korean Tissue Engineering and Regenerative Medicine Society 2020
Abstract BACKGROUND: Autophagy plays important roles in odontogenic differentiation of dental pulp cells (DPCs) in the developmental stage of tooth bud. Few studies have reported the role of autophagy during reparative dentin formation process. The objective of this study was to discover gene expression pattern correlated to autophagy and their role during odontogenic differentiation process in DPCs. METHODS: After tooth cavities were prepared on the mesial surface of lower first molar crown of rats. Odontogenic differentiation and reparative dentin formation were assessed based on detection of morphology change with hematoxylin and eosin staining. RESULTS: After tooth cavities were prepared on the mesial surface of lower first molar crown of rats, odontogenic differentiation and reparative dentin formation were assessed based on detection of morphology change with hematoxylin and eosin staining and dentin sialophosphoprotein (DSPP), whereas autophagy inhibitor 3-methyladenine (3MA) reversed. Results of quantitative polymerized chain reaction array of autophagosome formation related genes revealed that GABARAPL2 was prominently upregulated while expression of other ATG8 family members were moderately increased after tooth cavity preparation. In addition, human DPCs incubated in differentiation medium predominantly upregulated MAP1LC3C, which selectively decreased by 3MA but not by autophagy enhancer trehalose. Knock-down of MAP1LC3C using shRNA resulted in strong downregulation of dentin matrix protein 1 and DSPP as well-known odontogenic marker compared to knock-down of MAP1LC3B during odontogenic differentiation process of human DPCs. CONCLUSION: Our results suggest that MAP1LC3C plays a crucial role in odontogenic differentiation of human DPCs via regulating autophagic flux. Keywords Cell differentiation Odontogenesis Pulp biology Stem cells
Heui Seung Cho and Sam-Young Park contributed equally to this study.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13770-020-00310-3) contains supplementary material, which is available to authorized users. & Won Jae Kim [email protected] & Ji Yeon Jung [email protected]
1
Department of Oral Physiology, School of Dentistry, Hardtissue Biointerface Research Center, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of Korea
2
Department of Pediatric Dentistry, Dental Science Research Institute, School of Dentistry, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, Republic of Korea
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Tissue Eng Regen Med
1 Introduction During tooth eruption, dental papilla as part of the tooth germ contains mesen
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