The MYB transcription factor Baymax1 plays a critical role in rice male fertility
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ORIGINAL ARTICLE
The MYB transcription factor Baymax1 plays a critical role in rice male fertility Xiao‑Jiao Xiang1,2,3 · Lian‑Ping Sun1 · Ping Yu1 · Zheng‑Fu Yang1,2 · Pei‑Pei Zhang1 · Ying‑Xin Zhang1 · Wei‑Xun Wu1 · Dai‑Bo Chen1 · Xiao‑Deng Zhan1 · Riaz‑Muhammad Khan1 · Adil Abbas1 · Shi‑Hua Cheng1 · Li‑Yong Cao1 Received: 5 July 2020 / Accepted: 8 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Rice male fertility gene Baymax1, isolated through map-based cloning, encodes a MYB transcription factor and is essential for rice tapetum and microspore development. Abstract The mining and characterization of male fertility gene will provide theoretical and material basis for future rice production. In Arabidopsis, the development of male organ (namely anther), usually involves the coordination between MYB (v-myb avian myeloblastosis viral oncogene homolog) and bHLH (basic helix-loop-helix) members. However, the role of MYB proteins in rice anther development remains poorly understood. In this study, we isolated and characterized a male sterile mutant (with normal vegetative growth) of Baymax1 (BM1), which encodes a MYB protein. The bm1 mutant exhibited slightly lagging meiosis, aborted transition of the tapetum to a secretory type, premature tapetal degeneration, and abnormal pollen exine formation, leading to ultimately lacks of visible pollens in the mature white anthers. Map-based cloning, complementation and targeted mutagenesis using CRISPR/Cas9 technology demonstrated that the mutated LOC_Os04g39470 is the causal gene in bm1. BM1 is preferentially expressed in rice anthers from stage 5 to stage 10. Phylogenetic analysis indicated that rice BM1 and its homologs in millet, maize, rape, cabbage, and pigeonpea are evolutionarily conserved. BM1 can physically interacts with bHLH protein TIP2, EAT1, and PHD (plant homeodomain)-finger member TIP3, respectively. Moreover, BM1 affects the expression of several known genes related to tapetum and microspore development. Collectively, our results suggest that BM1 is one of key regulators for rice male fertility and may serve as a potential target for rice malesterile line breeding and hybrid seed production.
Communicated by Takuji Sasaki. Xiao-Jiao Xiang and Lian-Ping Sun contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03706-w) contains supplementary material, which is available to authorized users. * Shi‑Hua Cheng [email protected] * Li‑Yong Cao [email protected] 1
Key Laboratory for Zhejiang Super Rice Research and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China
2
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
3
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China
Abbreviations AD Activation domain BD Binding domain bHLH Basic helix-lo
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