Overexpression of wheat transcription factor ( TaHsfA6b ) provides thermotolerance in barley
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ORIGINAL ARTICLE
Overexpression of wheat transcription factor (TaHsfA6b) provides thermotolerance in barley Anuj Kumar Poonia1 · Sumit Kumar Mishra1 · Parul Sirohi1,2 · Reeku Chaudhary1 · Meenakshi Kanwar1 · Hugo Germain2 · Harsh Chauhan1 Received: 2 July 2020 / Accepted: 28 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Main conclusion Overexpressing a heat shock factor gene (TaHsfA6bT) from wheat provides thermotolerance in barley by constitutive expression of heat and other abiotic stress-response genes. Abstract Temperature is one of the most crucial abiotic factors defining the yield potential of temperate cereal crops, such as barley. The regulators of heat shock response (HSR), heat stress transcription factors (Hsfs), modulate the transcription level of heat-responsive genes to protect the plants from heat stress. In this study, an Hsf from wheat (TaHsfA6b) is overexpressed in barley for providing thermotolerance. Transgenic barley lines overexpressing TaHsfA6b showed improvement in thermotolerance. The constitutive overexpression of a TaHsfA6b gene upregulated the expression of major heat shock proteins and other abiotic stress-responsive genes. RNA-seq and qRT-PCR analysis confirmed the upregulation of Hsps, chaperonins, DNAJ, LEA protein genes, and genes related to anti-oxidative enzymes in transgenic lines. Excessive generation and accumulation of reactive oxygen species (ROS) occurred in wild-type (WT) plants during heat stress; however, the transgenic lines reflected improved ROS homeostasis mechanisms, showing lesser ROS accumulation under high temperature. No negative phenotypic changes were observed in overexpression lines. These results suggest that TaHsfA6b is a regulator of HSR and its overexpression altered the expression patterns of some main stress-related genes and enhanced the thermotolerance of this cereal crop. Keywords Heat stress · Heat stress factors · HSFA6 · Hordeum vulgare · Transgenic barley · Transcriptomics Abbreviations HS Heat stress HSR Heat stress response
Communicated by Dorothea Bartels. Anuj Kumar Poonia and Sumit Kumar Mishra are contributed equally in this study Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03457-4) contains supplementary material, which is available to authorized users. * Harsh Chauhan [email protected] 1
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India
Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, Trois‑Rivières, QC G9A 5H7, Canada
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HSF Heat stress transcription factor APX Ascorbate peroxidase CAT Catalase SOD Superoxide dismutase
Introduction Plants are sessile and, therefore, cannot escape the deleterious effects of heat stress (HS) that affect their growth, physiology, and development. HS impacts various physiological and metabolic processes, such as reactive oxygen species (ROS) production that leads to oxidative DNA damage, and pro
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