Paper-based electrochemiluminescence determination of streptavidin using reticular DNA-functionalized PtCu nanoframes an
- PDF / 2,117,387 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 60 Downloads / 162 Views
ORIGINAL PAPER
Paper-based electrochemiluminescence determination of streptavidin using reticular DNA-functionalized PtCu nanoframes and analyte-triggered DNA walker Yuzhen Huang 1,2 & Lina Zhang 3 & Sibao Zhang 4 & Peini Zhao 2 & Li Li 2 & Shenguang Ge 1
&
Jinghua Yu 2
Received: 5 May 2020 / Accepted: 18 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A paper-based electrochemiluminescence (ECL) biosensor characterized by the signal amplification of reticular DNAfunctionalized PtCu nanoframes (DNA-PtCuTNFs) and analyte-triggered DNA walker was developed for sensitive streptavidin assay. Silver microflower functionalized paper-based sensing platform was prepared to fix the hairpin strand (S1). With addition of the streptavidin, plenty of DNA walkers consisting of the walking strands (S2) labeled with biotin and streptavidin were established, which protected S2 from digestion via the terminal protection mechanism. The sequential introduction of the DNA walker and capture probe initiated the hairpin structure opening of S1 and strand displacement reaction (SDR) happening, causing the S2 release. Subsequently, S1 hybridized with S3. The free S2 further hybridized with adjacent S1 to trigger the next cycle. After multiple cycles, the DNA-PtCuTNFs, the fire-new signal enhancer, with remarkable peroxidase activity, were successfully attached onto the paper electrode via metal-catalyst-free click chemistry. Based on the SDR of the DNA walker and the catalysis of DNA-PtCuTNFs, a significantly boosted ECL signal of luminol was obtained. Under the optimal conditions, the developed sensor for streptavidin assay exhibited a low detection limit of 33.4 fM with a linear range from 0.1 pM to 0.1 μM. Keywords DNA walker . PtCu tripyramidal nanoframes . Electrochemiluminescence . Click chemistry
Introduction Proteins play a key role in most life activities. Especially, streptavidin (SA), a protein that is isolated from bacteria Streptomyces avidin, still maintains its activity in extreme environments. And this stable SA possesses the high affinity with biotin, promoting its application in medical, biological, and analysis fields. In order to play its role effectively,
ultrasensitive SA detection is a first essential step for obtaining high yield and purity of SA from Streptomyces avidin. Recently, many technologies have been developed for the detection of SA. For instance, Li et al. have firstly introduced the molecular translators into protein assay. Gold nanoparticles were used as the scaffold for binding-induced DNA strand displacement, achieving the fluorescent detection of SA successfully [1]. In order to improve the sensitivity,
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04515-0) contains supplementary material, which is available to authorized users. * Li Li [email protected]
2
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University
Data Loading...
